PMID: 3768347Sep 9, 1986Paper

A 59-kilodalton protein associated with progestin, estrogen, androgen, and glucocorticoid receptors

Biochemistry
P K TaiL E Faber

Abstract

Previous studies of the anti 8.5S progestin receptor monoclonal antibody KN 382/EC1 showed that it was specific for nontransformed progestin receptors. However, with different methods of tissue disruption and the use of protease inhibitors, we found that other nontransformed steroid receptors formed immune complexes with KN 382/EC1. Binding of the antibody to rabbit uterine estrogen, progestin, and androgen and liver glucocorticoid receptor systems was characterized by sucrose density gradient centrifugation, high-pressure liquid chromatography (HPLC), immunoadsorption, and immunoblotting. Immobilized KN 382/EC1 adsorbed both Mr 59,000 and Mr 92,000 proteins. The Mr 92,000 protein appeared to be bound to the antigenic Mr 59,000 protein, and the two proteins were present in apparently the same stoichiometric relationship in several tissues. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of immunoadsorbed material revealed appreciable amounts of both proteins in testis, stomach, lung, liver, uterus, and kidney. Only trace amounts were found in skeletal or heart muscle, and none was found in blood serum. Cleveland digestion of isolated Mr 59,000 and 92,000 proteins revealed dissimilar peptide constituents. Immunoblots o...Continue Reading

References

Jan 1, 1985·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·P K Tai, L E Faber
Jan 1, 1985·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·K NakaoL E Faber
Jan 1, 1972·Annual Review of Biochemistry·E V Jensen, E R DeSombre
Aug 1, 1984·Molecular and Cellular Endocrinology·J M Renoir, J Mester
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C RadanyiE E Baulieu

❮ Previous
Next ❯

Citations

Dec 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W B Pratt
Feb 1, 1993·Journal of Cellular Biochemistry·A Baniahmad, M J Tsai
Apr 1, 1995·Endocrine·V R MartinsP R Housley
Jan 1, 1988·Journal of Steroid Biochemistry·D M Boyle, L A van der Walt
Jan 1, 1989·Journal of Steroid Biochemistry·M A Carson-JuricaB W O'Malley
Jan 1, 1989·Journal of Steroid Biochemistry·E OrtíA Munck
Nov 1, 1989·Journal of Steroid Biochemistry·T DemuraT Koyanagi
May 1, 1989·Journal of Steroid Biochemistry·L DufreneE E Baulieu
Apr 1, 1990·Journal of Steroid Biochemistry·T RatajczakR Hähnel
Sep 1, 1990·Steroids·H C AtkinsonR Hähnel
Apr 16, 1993·Brain Research. Developmental Brain Research·S A TobetM J Baum
Feb 16, 1991·Biochimica Et Biophysica Acta·M Muller, R Renkawitz
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·M RexinU Gehring
Sep 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·T NemotoM Ota
Sep 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·C RadanyiE E Baulieu
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·W B PrattF C Dalman
Apr 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·U Gehring
Feb 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·Y M Ning, E R Sanchez
Jan 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·J M RenoirE E Baulieu
Jan 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·J JohnsonD Toft
Jun 5, 2003·Genomics·Jonathan G ScammellDonna L Valentine
Mar 16, 2000·Breast Cancer Research and Treatment·B K WardT Ratajczak
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·I CallebautJ P Mornon
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P RehbergerU Gehring
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D A PeattieM Benasutti
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M K BagchiB W O'Malley
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E S AlnemriG Litwack
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·C RadanyiE E Baulieu
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Segnitz, U Gehring
Dec 5, 2006·The Journal of Biological Chemistry·Weidong YongWeinian Shou
Nov 1, 1994·Journal of Immunoassay·K L LeachM R Deibel
Oct 1, 1992·The Journal of Cell Biology·M Perrot-ApplanatE Milgrom
Aug 1, 1994·The Journal of Cell Biology·X ShanT Mélèse
Oct 21, 2003·DNA and Cell Biology·Nelly MassolEtienne-Emile Baulieu
Dec 1, 1995·Molecular Biology of the Cell·S J Holley, K R Yamamoto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.