A 595 nanometer band-pass filter enhances the contrast of in situ hybridization signals on chromosome observed after biotin/avidin-alkaline phosphatase localization.

Stain Technology
H YasueS Muramatsu

Abstract

In situ hybridization with DNA probes labeled with biotin and detected by the avidin-alkaline phosphatase/5-bromo-chloroindoxyl phosphate-nitro blue tetrazolium system has been used to localize DNA sequences in chromosomes. To observe the hybridization signals, a phase contrast microscope has often been used because of the good visibility it provides. Use of a 595 nm band pass filter with the phase contrast microscope enhances signal contrast after in situ hybridization without reducing resolution.

References

Jun 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J G Gall, M L Pardue
Feb 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J G BaumanP van Duijn
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P R LangerD C Ward
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P R Langer-SaferD C Ward

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