A 627K variant in the PB2 protein of H9 subtype influenza virus in wild birds

Influenza and Other Respiratory Viruses
Ye GeHualan Chen

Abstract

Wild birds are gaining increasing attention as gene-mixing reservoirs for influenza viruses. To investigate the molecular properties of the viruses isolated and epidemiological analysis of H9N2 subtype AIV in wild birds, we studied samples obtained over two years (2014-2015) from wetlands in Anhui province, China. A total of 4534 samples were collected from migratory waterfowl in Anhui in 2014-2015, and 8 strains of H9 subtype AIV were isolated. Phylogenetic analysis showed different degrees of gene segment reassortment in H9 viruses between the Eurasian lineage and the North American lineage. Most importantly, two viruses harbored the E627K mutation in the polymerase PB2 (PB2) protein. This is the first report of the mutation of this virus from low pathogenicity to high pathogenicity in wild birds. The continued surveillance of wild birds, especially migratory birds, is important to provide early warning and control of AIV outbreaks. Our results highlight the high genetic diversity of AIV along the Eurasian-Australian migration flyway and the need for more extensive AIV surveillance in eastern China.

References

Mar 1, 1992·Microbiological Reviews·R G WebsterY Kawaoka
Aug 27, 2002·Nature Medicine·Sang Heui SeoRobert G Webster
Jul 29, 2006·Journal of Virology·Dmitriy ZamarinPeter Palese
May 9, 2009·The New England Journal of Medicine·Fatimah S DawoodTimothy M Uyeki
Apr 13, 2013·The New England Journal of Medicine·Rongbao GaoYuelong Shu
Apr 20, 2014·Science·George F Gao
Nov 12, 2014·Protein & Cell·Yipeng Sun, Jinhua Liu

❮ Previous
Next ❯

Citations

Sep 27, 2018·EFSA Journal·UNKNOWN European Food Safety AuthorityFrancesca Baldinelli

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
glycosylation

Software Mentioned

mage
DNAstar package
SeqMan

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.