PMID: 6407010Jun 1, 1983Paper

A 64-kilodalton membrane protein of Bacillus subtilis covered by secreting ribosomes

Proceedings of the National Academy of Sciences of the United States of America
S HoriuchiB D Davis

Abstract

The complexed (ribosome-bearing) membrane fraction of Bacillus subtilis contains several proteins (CM-proteins) that are virtually absent from the ribosome-free fraction and hence might be components of the apparatus of protein secretion. We have determined, by trypsin digestion and by labeling with a nonpenetrating reagent (diazoiodosulfanilic acid), the accessibility of four of these proteins on the two surfaces of the membrane, as exposed either in protoplasts or in inverted membrane vesicles. The 68-kilodalton protein is a transmembrane protein and the 45-kilodalton protein faces only the external surface, whereas the 31-kilodalton protein is inaccessible from either side. Of particular interest is the 64-kilodalton protein: it can be digested by trypsin, and can bind antibody, on the cytoplasmic surface, but only after the ribosomes have been released. This protein is thus evidently a component of the apparatus of protein secretion, closely covered by secreting ribosomes. Whether the other CM-proteins are also involved in protein secretion is uncertain.

References

Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W P SmithB D Davis
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K TalmadgeW Gilbert
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P Walter, G Blobel
Jan 1, 1982·The Journal of Cell Biology·D D SabatiniM Adesnik

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Citations

May 1, 1992·Journal of Biotechnology·R Freudl
Aug 29, 2001·FEMS Microbiology Reviews·K H van WelyA J Driessen
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M P CaulfieldB D Davis
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M P CaulfieldB D Davis
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J J Laffan, W Firshein
Jan 1, 1986·CRC Critical Reviews in Biochemistry·T A Rapoport
Dec 4, 1984·Biochemistry·Y Ohno-IwashitaW Wickner
Mar 1, 1993·Microbiological Reviews·M Simonen, I Palva

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