A 67 kDa non-hormone binding estradiol receptor is present in human mammary cancers

International Journal of Cancer. Journal International Du Cancer
G CastoriaF Auricchio

Abstract

The presence of large amounts of a 67 kDa estradiol receptor that does not bind hormone was observed in 8 to 37 human mammary tumors (34 malignant and 3 benign). This form of receptor was detected by its conversion to hormone binding receptor by an endogenous tyrosine kinase in vitro. All 8 tumors were malignant. In these, the incubation of cytosol with ATP was seen to cause a 1- to 5-fold increase in estradiol-specific binding sites. These sites bound estradiol with physiological affinity, and their appearance was associated with tyrosine phosphorylation of estradiol receptor. The enzyme converting the non-hormone binding receptor into the hormone binding receptor is largely present in cytosol and scarce in membranes. It has been extensively purified. It is a 67 kDa protein under denaturating conditions, binds calmodulin-Sepharose in a Ca2+-dependent manner, is stimulated by Ca2+ and calmodulin, phosphorylates exogenous actin, is activated by the estradiol-receptor complex. The enzyme interacts with antibodies directed against the carboxy-terminal and catalytic domains of c-src. Therefore, it is a putative new member of the large c-src-related kinase family. Human mammary cancers with significant amounts of 67 kDa non-hormone ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·W L McGuireS A Fuqua
Jul 9, 1992·International Journal of Cancer. Journal International Du Cancer·A MigliaccioF Auricchio
Apr 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·A MigliaccioF Auricchio
Jul 4, 1990·Journal of Steroid Biochemistry·H LahootiA Aakvaag
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P NicoteraS Orrenius
Aug 1, 1985·Cell·J M Bishop
Jan 1, 1987·Methods in Enzymology·F AuricchioM Di Domenico
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A MigliaccioF Auricchio
Feb 15, 1981·The Biochemical Journal·F AuricchioA Rotondi
Feb 23, 1993·Biochemistry·G CastoriaF Auricchio
Nov 1, 1994·International Journal of Cancer. Journal International Du Cancer·M A NagaiM M Brentani

❮ Previous
Next ❯

Citations

Jun 6, 2008·Molecular and Cellular Endocrinology·Dominique GalloGuy Leclercq
Apr 12, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Guy Leclercq

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.