PMID: 9443967Mar 14, 1998Paper

A 7-methylguanosine cap commits U3 and U8 small nuclear RNAs to the nucleolar localization pathway

Nucleic Acids Research
M R Jacobson, Thoru Pederson

Abstract

U3 and U8 small nucleolar RNAs (snRNAs) participate in pre-rRNA processing. Like the U1, U2, U4 and U5 major spliceosomal snRNAs, U3 and U8 RNAs are transcribed by RNA polymerase II and their initial 7-methylguanosine (m7G) 5' cap structures subsequently become converted to 2,2,7-trimethylguanosine. However, unlike the polymerase II transcribed spliceosomal snRNAs, which are exported to the cytoplasm for cap hypermethylation, U3 and U8 RNAs undergo cap hypermethylation within the nucleus. Human U3 and U8 RNAs with various cap structures were generated by in vitro transcription, fluorescently labeled and microinjected into nuclei of normal rat kidney (NRK) epithelial cells. When U3 and U8 RNAs containing a m7G cap were microinjected they became extensively localized in nucleoli. U3 and U8 RNAs containing alternative cap structures did not localize in nucleoli nor did U3 or U8 RNAs containing triphosphate 5'-termini. The nucleolar localization of m7G-capped U3 RNA was competed by co-microinjection into the nucleus of a 100-fold molar excess of dinucleotide m7GpppG but not by a 100-fold excess of ApppG dinucleotide. Although it was obviously not possible to assess formation of di- and trimethylguanosine caps on the microinjected U...Continue Reading

References

Apr 1, 1992·Experimental Cell Research·C WersigA Bindereif
Dec 1, 1992·Current Opinion in Cell Biology·E H Kislauskis, R H Singer
Sep 1, 1992·The Journal of Cell Biology·E IzaurraldeI W Mattaj
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J WangT Pederson
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A M Kleinschmidt, T Pederson
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·G W Zieve, R A Sauterer
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R Singh, R Reddy
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G R KunkelT Pederson
Dec 29, 1967·Science·B G Forget, S M Weissman
Jan 1, 1995·Annual Review of Biochemistry·E S Maxwell, M J Fournier
Jul 1, 1995·Trends in Biochemical Sciences·J P BachellerieM J Fournier
Apr 21, 1995·Cell·D St Johnston
May 13, 1994·Science·M P Terns, J E Dahlberg
Jan 1, 1993·Annual Review of Cell Biology·D L Spector
Oct 1, 1993·Genes & Development·M P TernsE Lund
Oct 1, 1993·The Journal of Cell Biology·J E Wilhelm, R D Vale
Dec 15, 1996·Experimental Cell Research·D L Spector
May 30, 1997·Cell·C M Smith, J A Steitz

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Citations

Jul 9, 1999·Molecular Biology of the Cell·A NarayananM P Terns
Feb 17, 2001·Molecular Biology of the Cell·I MelcákI Raska
Oct 8, 1998·Molecular Biology of the Cell·T S LangeS A Gerbi
Nov 10, 2000·Nucleic Acids Research·W A SpeckmannM P Terns
Jul 28, 2010·Cold Spring Harbor Perspectives in Biology·Thoru Pederson
Nov 26, 2010·Cold Spring Harbor Perspectives in Biology·Thoru Pederson
Aug 22, 2007·Molecular and Cellular Biology·Nicholas J WatkinsReinhard Lührmann
Feb 1, 2012·RNA·Yun S ChoiMichael T McManus
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·M R Jacobson, T Pederson
Feb 27, 2001·Nucleic Acids Research·T Pederson
Mar 22, 2000·The Journal of Cell Biology·T Pederson, J C Politz
Nov 26, 2015·Frontiers in Plant Science·Lloyd Loza-MullerEnrique Castano
Nov 25, 2003·The EMBO Journal·Luisa Di StefanoKristian Helin
Feb 3, 2006·International Journal of Dental Hygiene·C MårtenssonS Renvert
Dec 3, 2004·Molecular Cell·Séverine BoulonEdouard Bertrand
Sep 22, 1999·Molecular and Cellular Biology·P FortesI W Mattaj
Jan 5, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T Pederson
Jun 6, 1998·Journal of Molecular Biology·T Pederson

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