A 72 kDa heat shock protein is protective against the selective vulnerability of CA1 neurons and is essential for the tolerance exhibited by CA3 neurons in the hippocampus

Neuroscience
K Sato, N Matsuki

Abstract

The correlation between the expression of a 72 kDa heat shock protein and vulnerability of hippocampal CA1, CA3, and dentate gyrus regions to glutamate toxicity was investigated using a highly specific antisense oligonucleotide technique. Glutamate (1 mM, 15 min) caused region-dependent neuronal damage in cultured hippocampal slices 24 h after exposure and the most severe damage was observed in CA1. When slices were heat-shocked (43.5 degrees C, 30 min) before exposure to glutamate, neuronal damage in CA1 was attenuated. The strongest protection was observed when the interval between the heat shock and the exposure to glutamate was 3 days, which coincided with the maximal induction of a 72 kDa heat shock protein in neurons. When the expression of a 72 kDa heat shock protein was suppressed by the antisense oligonucleotide, the protective effect of the heat shock was completely inhibited. Glutamate itself also induced a 72 kDa heat shock protein in neurons, region-dependently, 24 h after the exposure. The signal of a 72 kDa heat shock protein in CA3 and dentate gyrus was significantly stronger than that in CA1. When the antisense oligonucleotide was applied, the damage in CA3 and dentate gyrus was exaggerated dose-dependently, an...Continue Reading

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G C LiW M Lee
Jun 1, 1992·The Journal of Cell Biology·R P BeckmannW J Welch
Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Dec 1, 1991·Neuron·G RordorfJ V Bonventre
May 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K KitagawaT Kamada
Feb 1, 1988·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·E OzyurtJ McCulloch
Sep 30, 1988·Science·M F BarbeW J Welch
Sep 20, 1984·The Journal of Comparative Neurology·J Zimmer, B H Gähwiler
Jan 1, 1994·Trends in Biochemical Sciences·F U HartlT Langer
Feb 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Ciechanover, A L Schwartz
Oct 15, 1993·Journal of Neuroscience Research·F M LongoF R Sharp
Jan 1, 1993·Annual Review of Cell Biology·C Georgopoulos, W J Welch
Jan 1, 1996·Developmental Neuroscience·E Gould, H A Cameron
Nov 18, 1996·Brain Research·K SatoN Matsuki
Mar 24, 1998·Brain Research Bulletin·G Gegelashvili, A Schousboe
Oct 19, 2000·Neuropathology : Official Journal of the Japanese Society of Neuropathology·T Kirino

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Citations

Jul 31, 2013·Cell Stress & Chaperones·Malgorzata KowalczykJan Kowalski
Oct 6, 2007·The International Journal of Neuroscience·Shuqiao YaoXiaoli Qi
Dec 6, 2006·Proteomics·Youra LeeDo Hee Lee
Aug 6, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M NickersonM Fleshner
Mar 24, 2006·American Journal of Physiology. Cell Physiology·Kittiporn PhanvijhitsiriEugene B Chang

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