PMID: 11607139Dec 15, 1990Paper

A 75-kDa polypeptide, located primarily at the plasma membrane of carrot cell-suspension cultures, is photoaffinity labeled by the calcium channel blocker LU 49888

Proceedings of the National Academy of Sciences of the United States of America
P ThuleauR Ranjeva

Abstract

Calcium channel blockers of the phenylalkylamine family bind specifically to membranes and inhibit calcium uptake in carrot protoplast. LU 49888, an azido derivative of phenylalkylamine, behaves as its unmodified homolog in terms of affinity and specificity and therefore allows us to probe the receptor by photoaffinity labeling. Upon UV irradiation, a 75-kDa peptide was specifically labeled. Incubation of microsomes with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, a zwitterionic detergent, led to the solubilization of the LU 49888-binding protein. Electrophoretic analysis under denaturing conditions and gel filtration of the solubilized "receptor-ligand" complex show a 75-kDa peptide mainly located at the plasma membrane. Consequently the LU 49888-binding protein in plants differs significantly from its animal counterpart by its size and may be a primary target for external signal molecules.

References

Oct 1, 1988·Trends in Neurosciences·K P CampbellA H Sharp
Jun 1, 1988·European Journal of Biochemistry·T Schneider, F Hofmann
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G R HicksT L Lomax
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P ThuleauR Ranjeva
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A M Jones, M A Venis

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Citations

Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·H CanutG Weber
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P ThuleauJ I Schroeder
Jan 1, 1993·Critical Reviews in Plant Sciences·B W Poovaiah, A S Reddy
May 23, 2018·International Journal of Molecular Sciences·Kjell De VrieseSteffen Vanneste

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