PMID: 9657144Jul 10, 1998Paper

A 9 A resolution X-ray crystallographic map of the large ribosomal subunit

Cell
N BanT A Steitz

Abstract

The 50S subunit of the ribosome catalyzes the peptidyl-transferase reaction of protein synthesis. We have generated X-ray crystallographic electron density maps of the large ribosomal subunit from Haloarcula marismortui at various resolutions up to 9 A using data from crystals that diffract to 3 A. Positioning a 20 A resolution EM image of these particles in the crystal lattice produced phases accurate enough to locate the bound heavy atoms in three derivatives using difference Fourier maps, thus demonstrating the correctness of the EM model and its placement in the unit cell. At 20 A resolution, the X-ray map is similar to the EM map; however, at 9 A it reveals long, continuous, but branched features whose shape, diameter, and right-handed twist are consistent with segments of double-helical RNA that crisscross the subunit.

References

Mar 25, 1979·Journal of Molecular Biology·K R Leonard, J A Lake
May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Feb 20, 1986·Nature·R A Milligan, P N Unwin
Jul 22, 1994·Journal of Molecular Biology·A Malhotra, S C Harvey
Jul 1, 1995·Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·Y Endo
Oct 1, 1995·Current Opinion in Structural Biology·M G Rossmann
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·F SchlünzenZ Berkovitch-Yellin
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J FrankS Srivastava
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·H F NollerC Merryman
Sep 13, 1996·Journal of Molecular Biology·K R LataJ Frank
Feb 28, 1998·Nucleic Acids Research·A VerschoorJ Frank
Jan 1, 1997·Methods in Enzymology·Axel T Brünger
Jan 1, 1997·Methods in Enzymology·A D Podjarny, A G Urzhumtsev

❮ Previous
Next ❯

Citations

Feb 4, 2010·Analytical and Bioanalytical Chemistry·Yinan Wei, Sylvia Daunert
Jun 23, 2005·Extremophiles : Life Under Extreme Conditions·Daqi TuThomas A Steitz
Dec 4, 2003·Journal of Structural Biology·N Jiménez-LozanoJ M Carazo
Jun 14, 2005·Trends in Biochemical Sciences·Peter B Moore, Thomas A Steitz
Feb 11, 2003·Analytical Biochemistry·Cecilia Arfvidsson, Karl Gustav Wahlund
Apr 6, 2001·Cell·B A Maguire, R A Zimmermann
Feb 19, 2000·Current Opinion in Biotechnology·O Byron, R J Gilbert
Apr 8, 2000·Current Opinion in Structural Biology·M van Heel
Oct 24, 2000·Current Opinion in Structural Biology·Y Tao, W Zhang
Apr 12, 2001·Current Opinion in Structural Biology·V Ramakrishnan, P B Moore
Jan 23, 1999·Current Opinion in Structural Biology·W G Scott
Feb 27, 1999·Current Opinion in Structural Biology·B HardestyG Kramer
May 14, 1999·Current Opinion in Structural Biology·R K Agrawal, J Frank
Dec 16, 2000·Trends in Biochemical Sciences·W Baumeister, A C Steven
Dec 7, 2002·Trends in Biochemical Sciences·Patrick Aloy, Robert B Russell
Aug 23, 2003·Trends in Biochemical Sciences·Thomas A Steitz, Peter B Moore
Jul 4, 2001·Nature Reviews. Molecular Cell Biology·D L Lafontaine, D Tollervey
Mar 18, 2009·The Journal of Biological Chemistry·Peter A MeyerJianhua Fu
Dec 10, 1998·Nucleic Acids Research·M SzymanskiV A Erdmann
May 15, 1999·Nucleic Acids Research·D E GillespieJ E Feagin
Dec 24, 1998·Nucleic Acids Research·D E Draper, L P Reynaldo
Nov 12, 2010·Cold Spring Harbor Perspectives in Biology·Peter B Moore, Thomas A Steitz
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·D E BrodersenV Ramakrishnan
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·J L HansenT A Steitz
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·A BashanA Yonath
Jun 12, 2012·Acta Crystallographica. Section D, Biological Crystallography·Vladimir Y LuninManfred W Baumstark
Nov 6, 2013·Acta Crystallographica. Section D, Biological Crystallography·Kiran KulkarniDavid Barford
Sep 10, 2009·Antimicrobial Agents and Chemotherapy·Güliz GürelPeter B Moore
Mar 23, 2011·Biochemistry. Biokhimii︠a︡·V A Kolb
Oct 7, 2003·Annual Review of Biochemistry·Peter B Moore, Thomas A Steitz
Jun 15, 2005·Annual Review of Biochemistry·James M Ogle, V Ramakrishnan
Feb 19, 2009·Journal of Biology·Peter B Moore
Jul 5, 2012·PloS One·Jean E FeaginRobin R Gutell
Apr 30, 2011·Science·Amie K BoalAmy C Rosenzweig

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.