A B-Cell Gene Signature Correlates With the Extent of Gluten-Induced Intestinal Injury in Celiac Disease

Cellular and Molecular Gastroenterology and Hepatology
Mitchell E GarberMarkku Mäki

Abstract

Celiac disease (CeD) provides an opportunity to study autoimmunity and the transition in immune cells as dietary gluten induces small intestinal lesions. Seventy-three celiac disease patients on a long-term, gluten-free diet ingested a known amount of gluten daily for 6 weeks. A peripheral blood sample and intestinal biopsy specimens were taken before and 6 weeks after initiating the gluten challenge. Biopsy results were reported on a continuous numeric scale that measured the villus-height-to-crypt-depth ratio to quantify gluten-induced intestinal injury. Pooled B and T cells were isolated from whole blood, and RNA was analyzed by DNA microarray looking for changes in peripheral B- and T-cell gene expression that correlated with changes in villus height to crypt depth, as patients maintained a relatively healthy intestinal mucosa or deteriorated in the face of a gluten challenge. Gluten-dependent intestinal damage from baseline to 6 weeks varied widely across all patients, ranging from no change to extensive damage. Genes differentially expressed in B cells correlated strongly with the extent of intestinal damage. A relative increase in B-cell gene expression correlated with a lack of sensitivity to gluten whereas their relati...Continue Reading

Citations

Feb 15, 2018·Gut·Jonas F LudvigssonDaniel A Leffler
Nov 20, 2018·Briefings in Bioinformatics·Eugenio Del PretePietro Liò
Nov 22, 2020·Nature Reviews. Gastroenterology & Hepatology·Laura KiveläKatri Lindfors
Dec 17, 2020·BMJ Open Gastroenterology·Hanna Gustafsson BragdeJan Söderman
Mar 6, 2021·Gastroenterology·Thomas LejeuneValérie Abadie

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Datasets Mentioned

BETA
GSE3982

Methods Mentioned

BETA
biopsy
in vitro transcription
enzyme-linked immunosorbent assay

Software Mentioned

R
Immgen data browser
Java
GenomeStudio
Excel
Immunological Genome Project ( Immgen ) data browser
Treeview

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