A Babesia bovis gene syntenic to Theileria parva p67 is expressed in blood and tick stage parasites

Veterinary Parasitology
Jeanne M FreemanDonald P Knowles

Abstract

Completion of the Babesia bovis (T2Bo strain) genome provides detailed data concerning the predicted proteome of this parasite, and allows for a bioinformatics approach to gene discovery. Comparative genomics of the hemoprotozoan parasites B. bovis and Theileria parva revealed a highly conserved syntenic block of genes flanking the p67 gene of T. parva, a sporozoite stage-specific vaccine candidate against East Coast fever (ECF). The syntenic gene in B. bovis, designated bov57, encodes a protein of limited amino acid sequence identity (11.8%) to p67. Monoclonal antibodies were produced against recombinant BOV57 and were used to demonstrate expression of BOV57 in merozoite and kinete stages of the T2Bo strain of B. bovis. Transcript levels of bov57 in kinetes were increased 100-fold in comparison to msa-1, a previously identified gene encoding an erythrocyte stage surface protein. Amino acid sequence comparisons between the T2Bo strain and two attenuated and virulent strains from Argentina and Australia revealed a high degree of sequence conservation in BOV57 among these geographically and pathogenically divergent isolates (97% amino acid sequence identity). Additional genomic comparisons show that the bov57 gene locus is also c...Continue Reading

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Citations

Jul 30, 2014·Parasitology·Monica Florin-ChristensenLeonhard Schnittger
Apr 7, 2012·Journal of Proteomics·Isabel MarcelinoAna Varela Coelho
Nov 10, 2012·BMC Genomics·Lowell S KappmeyerKelly A Brayton
Jun 21, 2011·Veterinary Parasitology·Carlos E Suarez, Susan Noh
Jul 3, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Takahiro IshizakiNaoaki Yokoyama

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