A Bacillus Spore-Based Display System for Bioremediation of Atrazine.

Applied and Environmental Microbiology
Hsin-Yeh HsiehGeorge C Stewart

Abstract

Owing to human activities, a large number of organic chemicals, including petroleum products, industrial solvents, pesticides, herbicides (including atrazine [ATR]), and pharmaceuticals, contaminate soil and aquatic environments. Remediation of these pollutants by conventional approaches is both technically and economically challenging. Bacillus endospores are highly resistant to most physical assaults and are capable of long-term persistence in soil. Spores can be engineered to express, on their surface, important enzymes for bioremediation purposes. We have developed a Bacillus thuringiensis spore platform system that can display a high density of proteins on the spore surface. The spore surface-tethered enzymes exhibit enhanced activity and stability relative to free enzymes in soil and water environments. In this study, we evaluated a B. thuringiensis spore display platform as a bioremediation tool against ATR. The Pseudomonas sp. strain ADP atzA determinant, an ATR chlorohydrolase important to the detoxification of ATR, was expressed as a fusion protein linked to the attachment domain of the BclA spore surface nap layer protein and expressed in B. thuringiensis Spores from this strain are decorated with AtzA N-terminally l...Continue Reading

References

Sep 1, 1987·Molecular & General Genetics : MGG·P HaimaG Venema
Jun 1, 1993·Applied and Environmental Microbiology·R T MandelbaumD L Allan
Jul 28, 1999·Applied Microbiology and Biotechnology·D A Newcombe, D E Crowley
Feb 8, 2002·Applied Microbiology and Biotechnology·L P WackettN Shapir
Jul 9, 2002·Molecular Microbiology·Patricia SylvestreMichèle Mock
Sep 25, 2002·Journal of Theoretical Biology·Lloyd Demetrius
Nov 27, 2002·Biochemistry·Jennifer L SeffernickLawrence P Wackett
Mar 6, 2003·Journal of Bacteriology·Christopher SteichenCharles L Turnbough
Nov 30, 2004·Clinical and Experimental Pharmacology & Physiology·T D SutherlandJ G Oakeshott
May 20, 2005·Journal of Bacteriology·Nir ShapirLawrence P Wackett
Apr 1, 1995·Applied and Environmental Microbiology·R T MandelbaumL P Wackett
Sep 14, 2006·International Journal of Occupational and Environmental Health·Jennifer Beth Sass, Aaron Colangelo
Feb 27, 2008·Journal of Applied Microbiology·M CarreraJ-L Sagripanti
Sep 3, 2008·Molecular Microbiology·Brian M Thompson, George C Stewart
Feb 10, 2009·Applied and Environmental Microbiology·Colin ScottJohn G Oakeshott
Jun 6, 2009·Journal of Microbiology and Biotechnology·Tae Jung ParkJae-Gu Pan
Mar 8, 2011·Analytical and Bioanalytical Chemistry·Leslie D KnechtSylvia Daunert
Jan 10, 2012·Applied Microbiology and Biotechnology·Eduardo ReáteguiLawrence P Wackett
Oct 9, 2012·Journal of Applied Microbiology·G WilliamsL Baillie
Mar 1, 2013·Chemical Society Reviews·Andreas Liese, Lutz Hilterhaus
Jan 17, 2014·Journal of Chemical Biology·Ahmad Abolpour HomaeiRoberto Stevanato
Jun 25, 2015·Microbiology Spectrum·Rachele Isticato, Ezio Ricca
Oct 30, 2015·Microbiology and Molecular Biology Reviews : MMBR·George C Stewart
Jan 8, 2017·Applied Microbiology and Biotechnology·He WangRuijin Yang
Jun 13, 2017·Journal of Molecular Microbiology and Biotechnology·Huayou ChenJinru Jia
Jan 13, 2018·Journal of Environmental Management·Babita SharmaPratyoosh Shukla

❮ Previous
Next ❯

Citations

Jan 3, 2021·Biotechnology Advances·Ezio RiccaRachele Isticato

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Toxicological Sciences : an Official Journal of the Society of Toxicology
Nikolay M FilipovPatrick L Crittenden
Applied and Environmental Microbiology
S F Petras, L E Casida
© 2021 Meta ULC. All rights reserved