PMID: 9555904Apr 29, 1998Paper

A Bacillus subtilis gene induced by cold shock encodes a membrane phospholipid desaturase

Journal of Bacteriology
Pablo S AguilarDiego de Mendoza

Abstract

Bacillus subtilis grown at 37 degrees C synthesizes saturated fatty acids with only traces of unsaturated fatty acids (UFAs). However, when cultures growing at 37 degrees C are transferred to 20 degrees C, UFA synthesis is induced. We report the identification and characterization of the gene encoding the fatty acid desaturase of B. subtilis. This gene, called des, was isolated by complementation of Escherichia coli strains with mutations in either of two different genes of UFA synthesis. The des gene encodes a polypeptide of 352 amino acid residues containing the three conserved histidine cluster motifs and two putative membrane-spanning domains characteristic of the membrane-bound desaturases of plants and cyanobacteria. Expression of the des gene in E. coli resulted in desaturation of palmitic acid moieties of the membrane phospholipids to give the novel mono-UFA cis-5-hexadecenoic acid, indicating that the B. subtilis des gene product is a delta5 acyl-lipid desaturase. The des gene was disrupted, and the resulting null mutant strains were unable to synthesize UFAs upon a shift to low growth temperatures. The des null mutant strain grew as well as its congenic parent at 20 or 37 degrees C but showed severely reduced survival...Continue Reading

References

May 1, 1987·Journal of Bacteriology·P G JonesF C Neidhardt
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 1, 1994·International Journal of Systematic Bacteriology·A M VialeR G Ferreyra
Mar 1, 1994·Molecular Microbiology·P G Jones, M Inouye
Nov 1, 1996·Archives of Microbiology·P Graumann, M A Marahiel
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen

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Citations

Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·E B Cahoon, J Shanklin
Jul 9, 2004·Molecular Genetics and Genomics : MGG·M NickelT Schweder
Nov 8, 2006·Molecular Microbiology·Pablo S Aguilar, Diego de Mendoza
Jun 22, 2007·Molecular Microbiology·Paola BisicchiaKevin M Devine
Jan 1, 2008·Journal of Biophysics·I DigelG M Artmann
Jun 29, 2010·Journal of Bacteriology·Jana BeranováIvo Konopásek
Aug 17, 2014·Applied and Environmental Microbiology·Holly E SaitoElizabeth M Fozo
May 14, 2014·Annual Review of Microbiology·Diego de Mendoza
Apr 28, 2001·Journal of Bacteriology·G E SchujmanD de Mendoza
Sep 7, 2001·Annual Review of Microbiology·J W Campbell, J E Cronan
Oct 26, 2002·Journal of Bacteriology·Carsten L BeckeringMohamed A Marahiel
Oct 16, 2004·Molecular Biology of the Cell·Babette SchadeDavid Y Thomas
Jun 9, 2004·European Journal of Biochemistry·Casper M JørgensenJan Martinussen
Feb 16, 2005·Archives of Microbiology·María C Mansilla, Diego de Mendoza
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ying-Jie LuCharles O Rock
May 6, 2003·Journal of Bacteriology·Silvia G AltabeDiego de Mendoza
Oct 23, 2008·Journal of Lipid Research·Yong-Mei Zhang, Charles O Rock
Aug 4, 2009·Applied and Environmental Microbiology·Rebecca-Ayme HughesTom Humphrey
Nov 2, 2014·Molecular BioSystems·Joris BeldMichael D Burkart
Mar 26, 2016·Frontiers in Microbiology·Qixia LuoHaichun Gao

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