A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator

Gene
S InouyeF I Tsuji

Abstract

The bacterial cloning vector, pGreenscript A, derived from the mutated Aequorea green fluorescent protein (GFP-S65A) gene, when expressed in E. coli produced colonies that showed yellow color under daylight and strong green fluorescence under long-wave ultraviolet light. The vector was used to select for inserted foreign genes based on the loss of the yellow color/green fluorescence of E. coli cells caused by the insertional inactivation of GFP production.

References

Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·R HeimR Y Tsien
Feb 23, 1995·Nature·R HeimR Y Tsien
Sep 5, 1994·FEBS Letters·S Inouye, F I Tsuji
Feb 11, 1994·Science·M ChalfieD C Prasher
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·H OgawaK Umesono
Mar 1, 1996·Nature Biotechnology·A CrameriW P Stemmer
Feb 1, 1995·Bio/technology·S DelagraveD C Youvan

❮ Previous
Next ❯

Citations

Jan 5, 2002·Current Opinion in Pharmacology·N J Emptage
Sep 23, 2006·Cancer Gene Therapy·Y IwasakiM Kitajima
Jan 27, 2009·Protein Engineering, Design & Selection : PEDS·E DikiciS Daunert
Mar 6, 2007·Development, Growth & Differentiation·Makoto MochiiIsshin Shikata
Jan 8, 2014·Analytical Biochemistry·Paul T WinnardVenu Raman
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·W AkamatsuH Okano
Apr 4, 2009·Journal of Microbiological Methods·Dea-Eun CheongGeun-Joong Kim
May 24, 2011·Analytical Biochemistry·Hiromi MiuraMasato Ohtsuka
Apr 18, 2013·Protein Science : a Publication of the Protein Society·Elizabeth B Speltz, Lynne Regan
Mar 29, 2008·Analytical Biochemistry·Hong Keun Park, Chaoyang Zeng
Mar 17, 2004·FEMS Microbiology Letters·Martin Iain BahlLars Hestbjerg Hansen
Mar 20, 2012·Analytical Biochemistry·Dea-Eun CheongGeun-Joong Kim
Jan 7, 2016·Development·Tadashi NomuraKatsuhiko Ono
Jul 2, 2003·Nature Biotechnology·Tadanori YamadaShun'ichi Kuroda
Dec 11, 2007·Applied Neuropsychology·George K Henry, Craig Enders
Jun 5, 2002·The Journal of Biological Chemistry·Marek NapieralaRobert D Wells
Jun 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Margarita Daniela Tafoya-RamírezBernardo Franco

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.