A Bacterial Effector Co-opts Calmodulin to Target the Plant Microtubule Network

Cell Host & Microbe
Ming GuoJames R Alfano

Abstract

The bacterial pathogen Pseudomonas syringae depends on effector proteins secreted by its type III secretion system for the pathogenesis of plants. The majority of these effector proteins are known suppressors of immunity, but their plant targets remain elusive. Using Arabidopsis thaliana as a model host, we report that the HopE1 effector uses the host calcium sensor, calmodulin (CaM), as a co-factor to target the microtubule-associated protein 65 (MAP65), an important component of the microtubule network. HopE1 interacted with MAP65 in a CaM-dependent manner, resulting in MAP65-GFP dissociation from microtubules. Transgenic Arabidopsis expressing HopE1 had reduced secretion of the immunity protein PR-1 compared to wild-type plants. Additionally, Arabidopsis map65-1 mutants were immune deficient and were more susceptible to P. syringae. Our results suggest a virulence strategy in which a pathogen effector is activated by host calmodulin to target MAP65 and the microtubule network, thereby inhibiting cell wall-based extracellular immunity.

References

Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J WolffS A Berkowitz
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S H Leppla
Nov 15, 1984·Nature·T Mitchison, M Kirschner
Mar 1, 1996·The Plant Journal : for Cell and Molecular Biology·L Adam, S C Somerville
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J HaseloffS Hodge
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Daigo TakemotoAdrienne R Hardham
Aug 30, 2003·Science·Feng ShaoRoger W Innes
Jan 21, 2004·The Plant Journal : for Cell and Molecular Biology·Huanquan ZhengIan Moore
Feb 6, 2004·The Plant Journal : for Cell and Molecular Biology·Yashitola JamirJames R Alfano
May 7, 2004·Applied and Environmental Microbiology·Brent L HouseMichael L Kahn
Oct 19, 2004·Biophysical Journal·Devrim Pesen, Jan H Hoh
Oct 29, 2004·Plant & Cell Physiology·Masayoshi NakamuraTakashi Hashimoto
Feb 4, 2005·Molecular Biology of the Cell·Isabelle LoïodiceP T Tran
Mar 1, 2005·FEBS Letters·Chan Young ParkMoo Je Cho
Aug 5, 2005·Annual Review of Phytopathology·Jane Glazebrook
Apr 11, 2006·Annual Review of Phytopathology·L C van LoonC M J Pieterse
May 4, 2006·Annual Review of Plant Biology·David W Ehrhardt, Sidney L Shaw
Jul 19, 2006·Journal of Cell Science·Andrei P SmertenkoPatrick J Hussey
Oct 3, 2006·Current Opinion in Plant Biology·Michiko Sasabe, Yasunori Machida
Apr 13, 2007·Cell Biology International·Yohann BouttéBéatrice Satiat-Jeunemaitre
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Monika KaldeScott C Peck
Aug 1, 2008·Molecular Biology of the Cell·Jérémie GaillardMarylin Vantard
Feb 24, 2009·Plant Physiology·Elena Bray SpethSheng Yang He
Apr 21, 2009·The Plant Cell·Elizabeth Faris CrowellSamantha Vernhettes
Jun 11, 2009·Plant Signaling & Behavior·Shuta Asai, Hirofumi Yoshioka
Aug 7, 2009·Molecular Plant-microbe Interactions : MPMI·Ming GuoJames R Alfano
May 18, 2010·Current Opinion in Plant Biology·Kenichi Tsuda, Fumiaki Katagiri
Aug 10, 2010·Cell·Claire E Walczak, Sidney L Shaw
Jan 14, 2011·Current Opinion in Microbiology·Anna Block, James R Alfano
Oct 14, 2011·Nature Reviews. Molecular Cell Biology·Justin M KollmanDavid A Agard
Nov 24, 2011·The International Journal of Biochemistry & Cell Biology·Hélène de ForgesFranck Perez
Feb 10, 2012·PLoS Pathogens·Amy Huei-Yi LeeDarrell Desveaux
Oct 23, 2012·Cell Host & Microbe·Daolong Dou, Jian-Min Zhou

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Citations

Jan 15, 2016·Cell Host & Microbe·Christopher J Staiger
May 25, 2016·Seminars in Cell & Developmental Biology·William Underwood
Nov 12, 2016·Plant & Cell Physiology·Xiaoyang ZhuJean-Philippe Galaud
Apr 7, 2017·Molecular Plant-microbe Interactions : MPMI
Feb 27, 2018·Nature Reviews. Microbiology·Xiu-Fang XinSheng Yang He
Mar 3, 2018·International Journal of Molecular Sciences·Didier AldonJean-Philippe Galaud
Dec 19, 2017·The New Phytologist·Eunsook ParkSavithramma P Dinesh-Kumar
Jul 6, 2018·Annual Review of Phytopathology·Jiejie Li, Christopher J Staiger
Jan 29, 2020·Molecular Plant-microbe Interactions : MPMI·Alan Collmer
Apr 21, 2020·Environmental Microbiology·Jun-Seob KimChoong-Min Ryu
Apr 16, 2019·Frontiers in Plant Science·Zunaira Afzal NaveedGul Shad Ali
Jul 22, 2019·Nature Communications·Yongliang ZhangSavithramma P Dinesh-Kumar
Feb 28, 2020·Journal of Cell Science·Liam ElliottCharlotte Kirchhelle
Oct 13, 2017·Molecular Plant-microbe Interactions : MPMI·Ronaldo J D DalioMarcos Machado
Oct 25, 2020·The New Phytologist·Claudia-Nicole MeisrimlerRichard J Morris
Dec 7, 2018·Molecular Plant·Fan YangJames R Alfano
Oct 26, 2020·Current Opinion in Plant Biology·Deepak D Bhandari, Federica Brandizzi
Aug 19, 2021·Molecular Cell·Adam OsinskiVincent S Tagliabracci
Aug 27, 2021·Biological Reviews of the Cambridge Philosophical Society·Vladimir Gorshkov, Ivan Tsers
Sep 28, 2021·Molecular Plant Pathology·Arya Bagus Boedi IswantoSang Hee Kim
Oct 18, 2021·The New Phytologist·Samuel EastmanMing Guo
Aug 1, 2020·Cell Host & Microbe·Liu XianAlberto P Macho

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