A bacterial toxin-antitoxin module is the origin of inter-bacterial and inter-kingdom effectors of Bartonella

PLoS Genetics
Alexander HarmsChristoph Dehio

Abstract

Host-targeting type IV secretion systems (T4SS) evolved from conjugative T4SS machineries that mediate interbacterial plasmid transfer. However, the origins of effectors secreted by these virulence devices have remained largely elusive. Previous work showed that some effectors exhibit homology to toxins of bacterial toxin-antitoxin modules, but the evolutionary trajectories underlying these ties had not been resolved. We previously reported that FicT toxins of FicTA toxin-antitoxin modules disrupt cellular DNA topology via their enzymatic FIC (filamentation induced by cAMP) domain. Intriguingly, the FIC domain of the FicT toxin VbhT of Bartonella schoenbuchensis is fused to a type IV secretion signal-the BID (Bep intracellular delivery) domain-similar to the Bartonella effector proteins (Beps) that are secreted into eukaryotic host cells via the host-targeting VirB T4SS. In this study, we show that the VbhT toxin is an interbacterial effector protein secreted via the conjugative Vbh T4SS that is closely related to the VirB T4SS and encoded by plasmid pVbh of B. schoenbuchensis. We therefore propose that the Vbh T4SS together with its effector VbhT represent an evolutionary missing link on a path that leads from a regular conjug...Continue Reading

References

Jul 1, 1995·Molecular Microbiology·R B Jensen, K Gerdes
Dec 1, 1996·Journal of Bacteriology·D Henderson, R J Meyer
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·T F Cooper, J A Heinemann
Aug 9, 2001·International Journal of Systematic and Evolutionary Microbiology·C DehioA Sander
Aug 10, 2002·Journal of Bacteriology·Lishan ChenEugene W Nester
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Michael F MinnickD Scott Samuels
Jan 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ralf SchuleinChristoph Dehio
Mar 5, 2005·Molecular Biology and Evolution·A Carolin FrankSiv G E Andersson
Aug 19, 2006·Journal of Bacteriology·Daniel Pérez-MendozaJuan Sanjuán
Aug 23, 2006·Journal of Bacteriology·Rembrandt J F HaftBeth Traxler
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Petr G LeimanJohn J Mekalanos
Jun 9, 2009·PloS One·Lisa N KinchNick V Grishin
May 28, 2010·Proceedings. Biological Sciences·Tim F CooperJack A Heinemann
Jun 16, 2010·PLoS Pathogens·Muriel Vayssier-TaussatChristoph Dehio
Nov 3, 2010·Current Opinion in Microbiology·Laurence Van Melderen
Jan 8, 2011·Protein Science : a Publication of the Protein Society·Dinesh V PalaniveluTilman Schirmer
Feb 22, 2011·Bioinformatics·Diego DarribaDavid Posada
Jul 6, 2011·Molecular Plant Pathology·Jennifer D LewisDarrell Desveaux
Sep 13, 2011·Journal of Bacteriology·Esther Fernández-GonzálezMatxalen Llosa
Oct 22, 2011·Mobile Genetic Elements·Nat F Brown, B Brett Finlay
Apr 26, 2013·Biology Letters·R Fredrik InglisMartin Ackermann
Oct 22, 2013·Nature Chemical Biology·Daniel Castro-RoaNikolay Zenkin
Jan 7, 2014·Frontiers in Plant Science·Andrea Pitzschke
May 9, 2014·Frontiers in Cellular and Infection Microbiology·Günther Koraimann, Maria A Wagner
Jul 22, 2014·Trends in Microbiology·Eric DurandLaure Journet

❮ Previous
Next ❯

Citations

Jan 1, 2020·Cellular Microbiology·Sophie BlevesMatxalen Llosa
May 26, 2020·BMC Microbiology·Francesca Romana CianfanelliDirk Bumann
Jun 6, 2019·Frontiers in Microbiology·Germán G SgroChuck S Farah
Jun 25, 2019·Cellular Microbiology·Maxime Québatte, Christoph Dehio
Sep 5, 2020·Annual Review of Genetics·Alejandro BurgaLeonid Kruglyak
Jan 12, 2021·Computational and Structural Biotechnology Journal·Cristina E Alvarez-MartinezChuck S Farah
Jan 2, 2021·Computational and Structural Biotechnology Journal·Si-Ping ZhangYong-Xing He
Jan 26, 2021·Current Opinion in Biotechnology·Joyati DasGopaljee Jha
Feb 2, 2021·Frontiers in Cellular and Infection Microbiology·Wolfgang EisenreichWerner Goebel
Apr 7, 2019·Microbiology Spectrum·Yang Grace LiPeter J Christie
Jan 4, 2022·Nature Reviews. Microbiology·Dukas JurėnasLaurence Van Melderen

❮ Previous
Next ❯

Datasets Mentioned

BETA
CP019790.1
CP000389.1

Methods Mentioned

BETA
PCR
FCS

Software Mentioned

MAFFT
UniParc
ProtTest
PhyML
BLAST
Phyre2
CRAfT
Geneious
PezTA
ClustalW

Related Concepts

Related Feeds

Bartonellosis

Bartonellosis is an infection caused by bacteria in the genus Bartonella and can present as a variety of diseases depending on the organism involved. While asymptomatic infection has been reported, immunocompromised individuals are more susceptible to severe infection. Find the latest research on bartonellosis here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cat-scratch disease

Cat-scratch disease, a relatively common infectious disease, is caused by Bartonella henselae and characterized by a fever and regional lymphadenitis. Here is the latest research.