PMID: 12770384May 29, 2003Paper

A baculovirus enhancin alters the permeability of a mucosal midgut peritrophic matrix from lepidopteran larvae

Journal of Insect Physiology
J PengR R Granados

Abstract

The peritrophic matrix (PM) in lepidopterous larvae may function as a defensive barrier against ingested viral pathogens. PMs isolated from Trichoplusia ni and Pseudaletia unipuncta larvae, were treated with a baculovirus-encoded metalloprotease (enhancin) from Trichoplusia ni granulosis virus (TnGV) and their in vitro permeability to blue dextran and fluorescent-labelled Autographa californica nuclear polyhedrosis virus (AcMNPV) was determined using a dual chamber permeability apparatus. Incubation of T. ni PMs with 0.0, 0.5, 1.0, and 2.0mg/ml enhancin resulted in a blue dextran 2000 flux of 4.4, 6.3, 9.9, and 15.6&mgr;g/mm(2)/h, respectively. In addition, T. ni PMs treated with enhancin were found to be significantly more permeable to fluorescent-labelled AcMNPV than non-treated control PMs. The permeability of T. ni PMs treated with 3.0mg/ml enhancin was 0.017 cumulative percent crossing/mm(2)/h, whereas the permeability of the control PM was below the detectable limit. Similarly, enhancin treatment greatly increased the permeability of P. unipuncta PMs to AcMNPV. These results provide evidence that the PM from two lepidopteran species can block the passage of baculovirions across this matrix thus reducing the probability of...Continue Reading

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Citations

May 29, 2001·Archives of Insect Biochemistry and Physiology·P Wang, R R Granados
Feb 13, 2003·Journal of Invertebrate Pathology·Wataru Mitsuhashi, Kazuhisa Miyamoto
Dec 11, 2008·Annual Review of Entomology·Dwayne HegedusUmut Toprak
Apr 7, 2006·The Journal of General and Applied Microbiology·Bibhuti R DasGupta
Sep 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tibor PechanDawn S Luthe
Feb 14, 2016·Developmental and Comparative Immunology·Kai WuErjun Ling
Apr 19, 2016·Archives of Insect Biochemistry and Physiology·Andres F Sandoval-Mojica, Michael E Scharf
Sep 26, 2006·Advances in Virus Research·Sijun LiuBryony C Bonning
Sep 10, 2003·International Reviews of Immunology·Thomas E Clarke, Rollie J Clem
May 1, 2010·Toxins·Robert L Harrison, Bryony C Bonning
Jul 30, 2005·Journal of Virology·James M Slavicek, Holly J R Popham
Sep 1, 2017·Archives of Virology·Ma de Los Ángeles Bivian-HernándezMaría Cristina Del Rincón-Castro
Jun 17, 2020·Journal of Chemical Ecology·Charles J Mason
Jul 14, 2019·Journal of Insect Physiology·Kotaro Konno, Wataru Mitsuhashi
Aug 20, 2020·Insect Biochemistry and Molecular Biology·Shaohua Wang, Ping Wang
Sep 2, 2008·Journal of Insect Physiology·Renata BolognesiClélia Ferreira
Apr 20, 2021·Annual Review of Virology·Enhao MaGong Cheng

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