A BAHD neofunctionalization promotes tetrahydroxycinnamoyl spermine accumulation in the pollen coat of the Asteraceae family

Journal of Experimental Botany
Marianne DelporteDavid Gagneul

Abstract

In eudicotyledons, accumulation of trihydroxycinnamoyl spermidine that is restricted to the pollen wall constitutes an evolutionary conserved trait. However, the role of this compound, which is synthetized by the BAHD enzyme spermidine hydroxycinnamoyl transferase (SHT), is still a matter of debate. Here, we show that this particular phenolamide is replaced by tetrahydroxycinnamoyl spermine in the pollen coat of the Asteraceae. Phylogenetic analyses combined with quantitative RT-PCR experiments allowed the identification of two homologous genes from Cichorium intybus (chicory) putatively involved in its metabolism. In vitro biochemical characterization of the two enzymes, named CiSHT1 and CiSHT2, confirmed the capability of recombinant proteins to synthesize spermine as well as spermidine derivatives. The wild-type metabolic phenotype was partially restored in an Arabidopsis sht mutant expressing CiSHT2. Strikingly, the transgenic plants also accumulated spermine derivatives that were absent in the wild-type. Overexpression of CiSHT2 in chicory hairy roots led to the accumulation of spermine derivatives, confirming its in vivo function. Complementary sequence analyses revealed the presence of an amino acid motif typical of the ...Continue Reading

References

Oct 25, 1988·Nucleic Acids Research·R Höfgen, L Willmitzer
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Feb 5, 2002·Chemical & Pharmaceutical Bulletin·Atsushi YamamotoMasanori Otsuka
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Apr 18, 2006·Current Opinion in Plant Biology·John C D'Auria
Jul 10, 2007·Natural Product Research·Shinji OhtaToshifumi Hirata
Apr 17, 2008·Journal of Agricultural and Food Chemistry·Victor S SobolevJames B Gloer
Apr 25, 2008·Plant Physiology and Biochemistry : PPB·Annalisa TassoniNello Bagni
Dec 17, 2008·The Plant Journal : for Cell and Molecular Biology·Etienne GrienenbergerMichel Legrand
May 22, 2009·Nucleic Acids Research·Timothy L BaileyWilliam S Noble
Jun 30, 2009·Phytochemistry·Maike PetersenStefan Wolters
Aug 31, 2010·Phytochemistry·Jean-Etienne BassardDanièle Werck-Reichhart
Apr 11, 2012·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Adriana EliasováMiroslav Repcák
Jun 30, 2012·Science·Jing-Ke WengJoseph P Noel
Dec 28, 2012·Cold Spring Harbor Symposia on Quantitative Biology·J-K Weng, J P Noel
May 3, 2014·Science·Lee ChaeSeung Y Rhee
Jun 8, 2014·Phytochemistry·Teagen D QuilichiniCarl J Douglas
Nov 20, 2014·Plant Physiology·Gregor Wolfgang SchmidtJohn Charles D'Auria
Dec 2, 2014·Trends in Plant Science·Tomas LaursenJean-Etienne Bassard
Mar 6, 2015·Trends in Plant Science·Christin Fellenberg, Thomas Vogt
Mar 1, 2016·Frontiers in Plant Science·Iris AloisiStefano Del Duca
Mar 17, 2016·Molecular Phylogenetics and Evolution·Jose L Panero, Bonnie S Crozier
Nov 4, 2016·Journal of Integrative Plant Biology·Kimiaki TanabeIvan Galis
Nov 8, 2017·Journal of Natural Products·Alen BožičevićMatthias Hamburger

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