A barley powdery mildew fungus non-autonomous retrotransposon encodes a peptide that supports penetration success on barley

Journal of Experimental Botany
Mathias NottensteinerRalph Hückelhoven

Abstract

Pathogens overcome plant immunity by means of secreted effectors. Host effector targets often act in pathogen defense, but might also support fungal accommodation or nutrition. The barley ROP GTPase HvRACB is involved in accommodation of fungal haustoria of the powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh) in barley epidermal cells. We found that HvRACB interacts with the ROP-interactive peptide 1 (ROPIP1) that is encoded on the active non-long terminal repeat retroelement Eg-R1 of Bgh. Overexpression of ROPIP1 in barley epidermal cells and host-induced post-transcriptional gene silencing (HIGS) of ROPIP1 suggested that ROPIP1 is involved in virulence of Bgh. Bimolecular fluorescence complementation and co-localization supported that ROPIP1 can interact with activated HvRACB in planta. We show that ROPIP1 is expressed by Bgh on barley and translocated into the cytoplasm of infected barley cells. ROPIP1 is recruited to microtubules upon co-expression of MICROTUBULE ASSOCIATED ROP GTPase ACTIVATING PROTEIN (HvMAGAP1) and can destabilize cortical microtubules. The data suggest that Bgh ROPIP targets HvRACB and manipulates host cell microtubule organization for facilitated host cell entry. This points to a possible neo...Continue Reading

References

Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·R T VoegeleK Mendgen
Aug 20, 2003·Acta Crystallographica. Section D, Biological Crystallography·Daniela DeriuMarkus G Grütter
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Holger SchultheissRalph Hückelhoven
Apr 30, 2004·Protein Engineering, Design & Selection : PEDS·Jannick Dyrløv BendtsenSøren Brunak
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Michael WalterJörg Kudla
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Aug 12, 2005·Cytogenetic and Genome Research·J JurkaJ Walichiewicz
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
May 10, 2008·Cellular Microbiology·Holger SchultheissRalph Hückelhoven
Jul 16, 2009·Molecular & Cellular Proteomics : MCP·Laurence V BindschedlerPietro D Spanu
Aug 7, 2010·Molecular Plant-microbe Interactions : MPMI·Ruth EichmannRalph Hückelhoven
Oct 7, 2011·Wiley Interdisciplinary Reviews. RNA·Dmitri A Kramerov, Nikita S Vassetzky
Jan 26, 2012·Nature Reviews. Immunology·Steven H Spoel, Xinnian Dong
Aug 7, 2012·Molecular Plant Pathology·Wen-Jing ZhangHans Thordal-Christensen
Dec 13, 2012·BMC Genomics·Carsten PedersenPietro D Spanu
Feb 28, 2013·Molecular Plant-microbe Interactions : MPMI·Clara PliegoPietro D Spanu
May 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Stéphane HacquardEmiel Ver Loren van Themaat
Jul 16, 2013·Nature Genetics·Thomas WickerBeat Keller
Apr 29, 2014·Molecular Cell·Alberto P Macho, Cyril Zipfel
May 30, 2014·Nature·Mathias WilhelmBernhard Kuster
Jul 9, 2014·Annual Review of Phytopathology·Chris C N van Schie, Frank L W Takken
Aug 30, 2014·The New Phytologist·Peter DörmannRalph Hückelhoven
Oct 30, 2014·Frontiers in Plant Science·Yoji KawanoKo Shimamoto
Apr 19, 2015·The Plant Cell·Amey RedkarGunther Doehlemann
Jan 28, 2016·Journal of Proteome Research·Helen G PenningtonLaurence V Bindschedler
Apr 9, 2016·Journal of Experimental Botany·Björn SchelerRalph Hückelhoven
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xunli LuPaul Schulze-Lefert

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Citations

May 28, 2019·The New Phytologist·Lamprinos FrantzeskakisRalph Panstruga
Apr 12, 2020·Cellular and Molecular Life Sciences : CMLS·Hans Thordal-Christensen
Jun 28, 2018·Journal of Experimental Botany·Björn Sabelleck, Ralph Panstruga
Sep 6, 2020·Cells·Stefan EngelhardtRalph Hückelhoven
Nov 30, 2020·Journal of Plant Physiology·Isabel M L Saur, Ralph Hückelhoven
Feb 2, 2021·Molecular Plant-microbe Interactions : MPMI·Soledad SacristánSebastian Eves-van den Akker
Oct 26, 2020·Current Opinion in Plant Biology·Deepak D Bhandari, Federica Brandizzi
Oct 10, 2021·Molecular Plant-microbe Interactions : MPMI

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