PMID: 9428677Jan 15, 1998Paper

A base-off analogue of coenzyme-B12 with a modified nucleotide loop--1H-NMR structure analysis and kinetic studies with (R)-methylmalonyl-CoA mutase, glycerol dehydratase, and diol dehydratase

European Journal of Biochemistry
L PoppeJ Rétey

Abstract

(Co beta-5'-Deoxyadenosin-5'-yl)-(p-cresolyl)cobamide (Ado-PCC), an analogue of the base-off form of coenzyme-B12 (CoB12), was prepared by alkylation of (Co alpha/beta-cyano/aqua)-(p-cresolyl)cobamide (PCC) with 5'-chloro-5'-deoxyadenosine. The 500 MHz 1H-NMR spectrum of Ado-PCC in D2O at pH 7.4 was completely analyzed using COSY and NOESY two-dimensional experiments. The coenzyme and inhibitory activities of Ado-PCC were tested with three coenzyme-B12-dependent enzymes: (R)-methylmalonyl-CoA mutase, glycerol dehydratase, and diol dehydratase. Ado-PCC showed strong coenzyme activity with methylmalonyl-CoA mutase, which is known to bind the base-off form of CoB12. In contrast, Ado-PCC had no coenzyme activity but acted instead as a competitive inhibitor with glycerol dehydratase and diol dehydratase, which are likely to prefer the base-on form of CoB12. These results indicate that Ado-PCC, whose structure is analogous to the base-off form of CoB12, can be used for probing the mode of coenzyme binding by coenzyme-B12-dependent enzymes.

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Citations

Oct 9, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Tetsuo Toraya
Jan 23, 1999·Current Opinion in Chemical Biology·P K Mishra, D G Drueckhammer
Sep 17, 2002·European Journal of Biochemistry·Mamoru YamanishiTetsuo Toraya
Feb 19, 2013·Journal of Bacteriology·Kenny C Mok, Michiko E Taga
Jun 24, 2011·Molecular Microbiology·Chi Ho Chan, Jorge C Escalante-Semerena
Jul 8, 2020·Science·Olga M SokolovskayaMichiko E Taga
Jun 9, 2005·Chemical Reviews·Kenneth L Brown
Sep 25, 1999·Archives of Biochemistry and Biophysics·A AbendG H Reed

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