A basic consideration for porcine liver preservation using a novel continuous machine perfusion device

Transplantation Proceedings
T ShigetaS Enosawa

Abstract

The aims of this study were to compare extracellular and intracellular-type University of Wisconsin (UW) solutions for liver grafts and to assess oxygenation in this perfusion system. The organ preservation system consisted of 3 circulating systems for the portal vein, hepatic artery, and maintenance of the perfusion solution. The portal vein or hepatic artery system had a roller pump, a flow meter, and a pressure sensor. In this study, we perfused livers with UW or extracellular type UW-gluconate at 4°C-6°C for 4 hours. The flow rates at the entrance were 0.5 mL/min/g liver in the portal vein and 0.2 mL/min/liver in the hepatic artery. Orthotopic liver transplantation was performed in pigs: group 1-a, grafts procured after acute hemorrhagic shock were preserved by a solution without O(2); group 1-b, grafts were preserved with O(2); group 2-a, grafts were perfused using intracellular type solution (UW); and group 2-b, grafts were perfused using extracellular-type solution (UW-gluconate). Effluent aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) levels in group 1-b were lower than those in group 1-a. Survival rates in group 2-a and group 2-b were 1/4 and 3/3, respectively. Effluent AST and LDH levels in the perfu...Continue Reading

References

Feb 18, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Thomas MinorFrank Dombrowski
Oct 21, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Maud BessemsThomas M van Gulik
Apr 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P DutkowskiP A Clavien
Oct 27, 2006·Annals of Biomedical Engineering·A van der PlaatsG Rakhorst
Jan 2, 2009·The New England Journal of Medicine·Cyril MoersRutger J Ploeg
Dec 5, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J V GuarreraJ C Emond

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Citations

Mar 5, 2016·British Journal of Anaesthesia·A G Bradbury, R E Clutton
Apr 29, 2014·Journal of Hepatology·Jay A Graham, James V Guarrera
May 1, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S A KarangwaR J Porte
Aug 14, 2015·World Journal of Gastroenterology : WJG·Jun-Jun JiaShu-Sen Zheng

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