A Bayesian adaptive basis algorithm for single particle reconstruction.

Journal of Structural Biology
Alp KucukelbirHemant D Tagare

Abstract

Traditional single particle reconstruction methods use either the Fourier or the delta function basis to represent the particle density map. This paper proposes a more flexible algorithm that adaptively chooses the basis based on the data. Because the basis adapts to the data, the reconstruction resolution and signal-to-noise ratio (SNR) is improved compared to a reconstruction with a fixed basis. Moreover, the algorithm automatically masks the particle, thereby separating it from the background. This eliminates the need for ad hoc filtering or masking in the refinement loop. The algorithm is formulated in a Bayesian maximum-a-posteriori framework and uses an efficient optimization algorithm for the maximization. Evaluations using simulated and actual cryogenic electron microscopy data show resolution and SNR improvements as well as the effective masking of particle from background.

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Citations

Jun 7, 2015·Journal of Structural Biology·Hemant D TagareMurali Rao
Feb 4, 2016·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Chenxi Huang, Hemant D Tagare
Jul 21, 2015·Journal of Structural Biology·Toshio MoriyaUlla Ruotsalainen
Apr 4, 2015·Journal of Structural Biology·Nicha C DvornekHemant D Tagare
Jan 10, 2018·BioMed Research International·C O S SorzanoJ M Carazo

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