A Bhas 42 cell transformation assay on 98 chemicals: the characteristics and performance for the prediction of chemical carcinogenicity

Mutation Research
Ayako SakaiNoriho Tanaka

Abstract

The Bhas 42 cell transformation assay is a short-term system using a clone of the BALB/c 3T3 cells transfected with an oncogenic murine ras gene (v-Ha-ras). The assay has previously been reported to be capable of detecting the tumor-initiating and tumor-promoting activities of chemical carcinogens according to the different protocols, an initiation assay and a promotion assay, respectively. We applied this short-term assay to 98 chemicals to characterize the assay and evaluate its performance for the detection of chemical carcinogenicity. When the assay results were compared with the existing genotoxicity data, the Bhas 42 cell transformation assay could detect a considerable number of Ames-negative and Ames-discordant carcinogens: and the promotion assay detected most of those Ames-negative and -discordant carcinogens. This fact suggested that the Bhas 42 cells behaved as initiated cells in the transformation assay. The performance indices were calculated from the assay results of 52 carcinogens and 37 non-carcinogens. The concordance was 78%, sensitivity 73%, specificity 84%, positive predictivity 86%, negative predictivity 69%, false negative 27% and false positive 16%. Of these values, the concordance, specificity, negative...Continue Reading

References

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Citations

Oct 26, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Kohji YamakageMasakazu Furuta
Mar 1, 2016·Environmental and Molecular Mutagenesis·Sung Gu HanC Gary Gairola
Dec 3, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Monica VaccariAnnamaria Colacci
Mar 25, 2015·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Kiyoshi SasakiNoriho Tanaka
Dec 7, 2011·Critical Reviews in Toxicology·Tatyana Y DoktorovaVera Rogiers
Mar 9, 2019·Environmental and Molecular Mutagenesis·Sung-Hee HwangMichael Lee
Jul 23, 2011·Alternatives to Laboratory Animals : ATLA·Damien BrehenyStephen P Faux
Jun 21, 2013·Alternatives to Laboratory Animals : ATLA·Dirk WeisenseeWalter K Schlage
Apr 5, 2017·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Yasunobu Aoki

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