A bibenzyl from Dendrobium ellipsophyllum induces apoptosis in human lung cancer cells

Journal of Natural Medicines
Anirut HlosrichokChatchai Chaotham

Abstract

Failure of current chemotherapeutic drugs leads to the recurrence of tumor pathology and mortality in lung cancer patients. This study aimed to evaluate the anticancer activity and related mechanisms of 4,5,4'-trihydroxy-3,3'-dimethoxybibenzyl (TDB), a bibenzyl extracted from Dendrobium ellipsophyllum Tang and Wang, in human lung cancer cells. Cytotoxicity of TDB (0-300 µM) in different types of human lung cancer cells (H460, H292 and H23) and human dermal papilla cells (DPCs) was evaluated via MTT viability assay. Selective anticancer activity of TDB against human lung cancer cells was demonstrated with a high IC50 (approximately > 300 µM) in DPCs, while IC50 in human lung cancer H460, H292 and H23 cells was approximately 100 ± 5.18, 100 ± 8.73 and 188.89 ± 8.30 µM, respectively. After treatment with 50 µM of TDB for 24 h, flow cytometry analysis revealed the significant increase of early and late apoptosis with absence of necrosis cell death in human lung cancer cells. The up-regulation of p53, a tumor-suppressor protein, was elucidated in human lung cancer cells treated with 10-50 µM of TDB. Alteration to down-stream signaling of p53 including activation of pro-apoptosis protein (Bcl-2-associated X protein; Bax), reduction o...Continue Reading

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Citations

Aug 26, 2018·International Journal of Cosmetic Science·Alka MadaanManu Jaggi
Jul 12, 2019·Phytotherapy Research : PTR·Onsurang WattanathamsanVarisa Pongrakhananon
Jul 20, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry· IksenVarisa Pongrakhananon
Aug 6, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Pornchanok TaweecheepChatchai Chaotham
Jul 24, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jyoti SinghAbha Meena

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Methods Mentioned

BETA
protein assay
flow cytometry

Software Mentioned

PC densitometry
analyst
CellQuest

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