A bicistronic DNA vaccine containing apical membrane antigen 1 and merozoite surface protein 4/5 can prime humoral and cellular immune responses and partially protect mice against virulent Plasmodium chabaudi adami DS malaria

Infection and Immunity
A RainczukP Smooker

Abstract

The ultimate malaria vaccine will require the delivery of multiple antigens from different stages of the complex malaria life cycle. In order to efficiently deliver multiple antigens with use of DNA vaccine technology, new antigen delivery systems must be assessed. This study utilized a bicistronic vector construct, containing an internal ribosome entry site, expressing a combination of malarial candidate antigens: merozoite surface protein 4/5 (MSP4/5) (fused to a monocyte chemotactic protein 3 chemoattractant sequence) and apical membrane antigen 1 (AMA-1) (fused to a tissue plasminogen activator secretion signal). Transfection of COS 7 cells with bicistronic plasmids resulted in production and secretion of both AMA-1 and MSP4/5 in vitro. Vaccination of BALB/c mice via intraepidermal gene gun and intramuscular routes against AMA-1 and MSP4/5 resulted in antibody production and significant in vitro proliferation of splenocytes stimulated by both AMA-1 and MSP4/5. Survival of BALB/c mice vaccinated with bicistronic constructs after lethal Plasmodium chabaudi adami DS erythrocytic-stage challenge was variable, although significant increases in survival and reductions in peak parasitemia were observed in several challenge trials ...Continue Reading

References

Aug 17, 1999·Current Opinion in Immunology·M F Good, D L Doolan
Oct 6, 1999·Current Opinion in Biotechnology·E Martínez-Salas
Nov 7, 1999·Molecular and Biochemical Parasitology·T WuR L Coppel
Mar 29, 2001·Nature Immunology·C R Mackay
Jun 14, 2001·International Journal for Parasitology·D L Doolan, S L Hoffman
Feb 8, 2002·Nature·Louis H MillerOgobara K Doumbo
Feb 21, 2002·Trends in Parasitology·Sanjai KumarStephen L Hoffman
Mar 22, 2002·Nature Reviews. Immunology·M F Good
Jun 13, 2002·Chemical Immunology·Sanjai KumarThomas L Richie
Jun 13, 2002·Chemical Immunology·Denise L Doolan, Stephen L Hoffman
Aug 23, 2002·Current Gene Therapy·P de Felipe

❮ Previous
Next ❯

Citations

Sep 19, 2006·Parasitology·A GomezM A Patarroyo
Apr 16, 2010·Medical Mycology·Pedro Antônio Castelo TeixeiraLeila M Lopes-Bezerra
Jul 24, 2015·Acta Parasitologica·Halina Wedrychowicz
Jun 6, 2009·Current Opinion in Immunology·Sofia Casares, Thomas L Richie
Jul 16, 2014·Molecular & Cellular Proteomics : MCP·Olivia C FinneyRuobing Wang
Oct 1, 2010·Critical Reviews in Biotechnology·Clarence M OngkudonMichael K Danquah
Jun 25, 2015·Microbiology Spectrum·Duarte Miguel F Prazeres, Gabriel A Monteiro
Jul 25, 2019·Journal of Proteome Research·Melanie J ShearsPhotini Sinnis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.