A bicistronic lentiviral vector based on the 1D/2A sequence of foot-and-mouth disease virus expresses proteins stoichiometrically

Journal of Biotechnology
Viviana TorresPedro Zamorano

Abstract

Classic IRES sequences are notorious for exerting biased expression in favor of upstream coding regions when placed into polycistronic vectors. Here, we report the development of a bicistronic lentiviral system based on the 1D/2A sequence from the foot-and-mouth disease virus that is able to maintain tightly balanced control of upstream and downstream protein expression for several days at a stoichiometry very closely approaching 1.0. Our results suggest that the 1D/2A sequence can be optimized in an FUGW lentiviral setting to coordinate expression of multiple polypeptides, presenting a potentially valuable tool to signaling network researchers and to the gene therapy community.

Citations

Dec 19, 2012·Metabolic Engineering·Melissa Brazier-Hicks, Robert Edwards
Dec 25, 2015·AMB Express·Juliana de Amorim AraújoFernando Araripe Gonçalves Torres
Oct 18, 2015·Methods : a Companion to Methods in Enzymology·Susanne HasseMihail Sarov
Apr 5, 2018·FEMS Yeast Research·Tatiana M Souza-MoreiraAnastasia Krivoruchko
Jul 7, 2020·Current Protocols in Stem Cell Biology·Rabea Dettmer, Ortwin Naujok

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