A Binary Bivalent Supramolecular Assembly Platform Based on Cucurbit[8]uril and Dimeric Adapter Protein 14-3-3

Angewandte Chemie
Pim J de VinkChristian Ottmann

Abstract

Interactions between proteins frequently involve recognition sequences based on multivalent binding events. Dimeric 14-3-3 adapter proteins are a prominent example and typically bind partner proteins in a phosphorylation-dependent mono- or bivalent manner. Herein we describe the development of a cucurbit[8]uril (Q8)-based supramolecular system, which in conjunction with the 14-3-3 protein dimer acts as a binary and bivalent protein assembly platform. We fused the phenylalanine-glycine-glycine (FGG) tripeptide motif to the N-terminus of the 14-3-3-binding epitope of the estrogen receptor α (ERα) for selective binding to Q8. Q8-induced dimerization of the ERα epitope augmented its affinity towards 14-3-3 through a binary bivalent binding mode. The crystal structure of the Q8-induced ternary complex revealed molecular insight into the multiple supramolecular interactions between the protein, the peptide, and Q8.

References

May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·A LevchenkoP W Sternberg
Apr 24, 2007·Angewandte Chemie·Li ZhangLuc Brunsveld
Mar 12, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Pedro Montes-NavajasHermenegildo Garcia
Jun 28, 2008·Journal of the American Chemical Society·Seiji Sakamoto, Kazuaki Kudo
Dec 30, 2009·Angewandte Chemie·Hoang D NguyenLuc Brunsveld
Feb 10, 2010·The Biochemical Journal·Catherine JohnsonCarol MacKintosh
May 13, 2010·Chemical Society Reviews·Dana A UhlenheuerLuc Brunsveld
Apr 9, 2011·Journal of the American Chemical Society·Jordan M ChinaiAdam R Urbach
Sep 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jing ZhaoHaian Fu
Jun 22, 2012·Nature Chemistry·Róise E McGovernPeter B Crowley
Sep 7, 2012·Angewandte Chemie·Carlo FastingRainer Haag
Dec 6, 2012·ACS Chemical Biology·Lech-Gustav MilroyChristian Ottmann
Feb 21, 2013·Nature Chemistry·David BierChristian Ottmann
May 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ingrid J De Vries-van LeeuwenAlbertus H de Boer
Apr 17, 2014·Chemical Reviews·Lech-Gustav MilroyChristian Ottmann
Jul 30, 2014·Chemical Communications : Chem Comm·Róise E McGovernPeter B Crowley
Sep 23, 2014·Chemistry & Biology·Michelle R ArkinJames A Wells
Oct 13, 2015·Drug Discovery Today·Yasaman Aghazadeh, Vassilios Papadopoulos
Nov 6, 2015·Angewandte Chemie·Lech-Gustav MilroyChristian Ottmann
Jan 26, 2016·Chembiochem : a European Journal of Chemical Biology·Michal JewginskiCameron David Mackereth
Feb 19, 2016·Proceedings of the National Academy of Sciences of the United States of America·Loes M SteversChristian Ottmann
Jun 30, 2016·Chemical Communications : Chem Comm·Sarah H Hewitt, Andrew J Wilson
Jun 30, 2016·Angewandte Chemie·Ralph P G BosmansLuc Brunsveld
Nov 30, 2016·Chembiochem : a European Journal of Chemical Biology·Anniek den HamerLuc Brunsveld

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Citations

Aug 25, 2017·Chemical Communications : Chem Comm·Javier Montenegro, Robert J Phipps
Mar 31, 2018·Chemistry : a European Journal·Kondalarao Kotturi, Eric Masson
Apr 20, 2018·Angewandte Chemie·Francesca GuagniniPeter B Crowley
Nov 11, 2017·Chemistry : a European Journal·Aishling M DoolanPeter B Crowley
Jun 15, 2018·Chemistry : a European Journal·Gao LiYan-Mei Li
Sep 1, 2018·Small·Hua ZouXia Wang
Oct 10, 2018·Chembiochem : a European Journal of Chemical Biology·Dung T DangLuc Brunsveld
Jul 10, 2019·Chemistry : a European Journal·Hang YinDavid Bardelang
Aug 29, 2019·Chemical Communications : Chem Comm·Jimi M AlexPeter B Crowley
Dec 12, 2019·Chemical Communications : Chem Comm·Francesca GuagniniPeter B Crowley
Oct 3, 2020·Acta Crystallographica. Section F, Structural Biology Communications·Alice BalloneChristian Ottmann
Jun 25, 2020·Nature Communications·Anthony J QuartararoBradley L Pentelute
Jan 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Linlu ZhaoJunqiu Liu
Aug 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luigi Di Costanzo, Silvano Geremia
Apr 19, 2019·Chemical Science·Pim J de VinkLuc Brunsveld
Aug 29, 2020·Angewandte Chemie·Yao-Hua LiuYu Liu
Oct 20, 2020·Angewandte Chemie·Jinqiao Dong, Anthony P Davis
Jan 21, 2021·Journal of the American Chemical Society·Kiefer O RambergPeter B Crowley
Jan 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joana N MartinsNuno Basílio
Jul 25, 2018·Current Opinion in Chemical Biology·Joel A Finbloom, Matthew B Francis
Mar 10, 2021·Nature Communications·Annika MeinersShirley K Knauer
May 27, 2021·Chemical Communications : Chem Comm·Gao LiYan-Mei Li
Jun 2, 2021·The Journal of Organic Chemistry·Rita J FernandesNuno Basílio
May 28, 2021·Chemical Science·Pim J de VinkLuc Brunsveld
Aug 6, 2021·Chemical Science·Héctor Barbero, Eric Masson
Sep 20, 2017·Journal of the American Chemical Society·Sam van DunLuc Brunsveld
Oct 19, 2017·Journal of the American Chemical Society·David BierChristian Ottmann
Aug 25, 2021·Journal of the American Chemical Society·Xavier GuilloryChristian Ottmann
Apr 7, 2020·Journal of the American Chemical Society·Zehuan HuangOren A Scherman
Oct 10, 2018·Journal of the American Chemical Society·Loes M SteversLuc Brunsveld
Aug 26, 2021·Chemistry : a European Journal·Kiefer O RambergPeter B Crowley
Nov 30, 2021·Chemical Reviews·Luis CruzFernando Pina

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