A bioactive hybrid three-dimensional tissue-engineering construct for cartilage repair

Journal of Biomaterials Applications
Mari AinolaYrjö T Konttinen

Abstract

The aim was to develop a hybrid three-dimensional-tissue engineering construct for chondrogenesis. The hypothesis was that they support chondrogenesis. A biodegradable, highly porous polycaprolactone-grate was produced by solid freeform fabrication. The polycaprolactone support was coated with a chitosan/polyethylene oxide nanofibre sheet produced by electrospinning. Transforming growth factor-β3-induced chondrogenesis was followed using the following markers: sex determining region Y/-box 9, runt-related transcription factor 2 and collagen II and X in quantitative real-time polymerase chain reaction, histology and immunostaining. A polycaprolactone-grate and an optimized chitosan/polyethylene oxide nanofibre sheet supported cellular aggregation, chondrogenesis and matrix formation. In tissue engineering constructs, the sheets were seeded first with mesenchymal stem cells and then piled up according to the lasagne principle. The advantages of such a construct are (1) the cells do not need to migrate to the tissue engineering construct and therefore pore size and interconnectivity problems are omitted and (2) the cell-tight nanofibre sheet and collagen-fibre network mimic a cell culture platform for mesenchymal stem cells/chondr...Continue Reading

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Citations

Jun 25, 2016·Expert Opinion on Therapeutic Patents·Elena López-RuizMacarena Perán
Jun 18, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wilhelmina Margaretha GroenJos Malda
Jan 26, 2016·Materials·Muhammad ZafarFarshid Sefat
Aug 5, 2017·Materials·Shehriar HusainSaad B Qasim

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Methods Mentioned

BETA
Fluorescence
PCR

Software Mentioned

OriginPro
Oligomer
Primer3
Soft Analysis System
AnalySis Pro

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