A bioactive peptide amidating enzyme is required for ciliogenesis

ELife
Dhivya KumarBetty A Eipper

Abstract

The pathways controlling cilium biogenesis in different cell types have not been fully elucidated. We recently identified peptidylglycine α-amidating monooxygenase (PAM), an enzyme required for generating amidated bioactive signaling peptides, inChlamydomonasand mammalian cilia. Here, we show that PAM is required for the normal assembly of motile and primary cilia inChlamydomonas, planaria and mice.ChlamydomonasPAM knockdown lines failed to assemble cilia beyond the transition zone, had abnormal Golgi architecture and altered levels of cilia assembly components. Decreased PAM gene expression reduced motile ciliary density on the ventral surface of planaria and resulted in the appearance of cytosolic axonemes lacking a ciliary membrane. The architecture of primary cilia on neuroepithelial cells inPam-/-mouse embryos was also aberrant. Our data suggest that PAM activity and alterations in post-Golgi trafficking contribute to the observed ciliogenesis defects and provide an unanticipated, highly conserved link between PAM, amidation and ciliary assembly.

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K L Kindle
Mar 1, 1990·Cell and Tissue Research·E Boisvieux-UlrichD Sandoz
May 5, 1997·The Journal of Cell Biology·T Kato-MinouraR Kamiya
Jan 1, 1997·Methods in Enzymology·A S KolhekarB A Eipper
Feb 17, 1998·Biochemical and Biophysical Research Communications·K Haller, S Fabry
Apr 1, 2000·The Plant Journal : for Cell and Molecular Biology·M SchrodaC F Beck
Aug 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Phillip A NewmarkAlejandro Sánchez Alvarado
Sep 29, 2004·Biochemistry·David J MerklerMitchell E Johnson
Jan 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Gáspár Jékely, Detlev Arendt
Aug 8, 2006·Cell Metabolism·Bryce A MendelsohnJonathan D Gitlin
Feb 14, 2007·The Journal of Cell Biology·Karl-Ferdinand Lechtreck, George B Witman
May 10, 2007·Journal of Cell Science·Jiehong PanSteven L Brody
Sep 21, 2007·Nucleic Acids Research·Sofia M C RobbAlejandro Sánchez Alvarado
Oct 24, 2007·Nature Reviews. Molecular Cell Biology·Manfred FliegaufHeymut Omran
Feb 6, 2008·The Journal of Cell Biology·Karl-Ferdinand LechtreckGeorge B Witman
Dec 5, 2008·The Plant Journal : for Cell and Molecular Biology·Attila MolnarDavid Baulcombe
Mar 20, 2009·Molecular Biology of the Cell·Xiaofeng ZuoJoshua H Lipschutz
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Maxence V NachuryHua Jin
Jan 1, 2009·Methods in Cell Biology·Panteleimon RompolasStephen M King
Apr 29, 2010·Journal of Experimental Botany·Eric HummelChris Hawes
Apr 30, 2010·Journal of Cell Science·Anahi Molla-HermanAlexandre Benmerah
Sep 17, 2010·Molecular Biology of the Cell·Panteleimon RompolasStephen M King
Jan 7, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Aoife M Waters, Philip L Beales
Jan 25, 2011·Nature Genetics·Erica E DavisNicholas Katsanis
Feb 12, 2011·The Plant Journal : for Cell and Molecular Biology·Janette KropatDavin Malasarn
Mar 26, 2011·Neuron·Angeliki Louvi, Elizabeth A Grove

❮ Previous
Next ❯

Citations

Jan 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Daniela StrenkertSabeeha S Merchant
Dec 7, 2019·PLoS Biology·Raj LuxmiBetty A Eipper
Mar 18, 2019·Cellular and Molecular Life Sciences : CMLS·Dhivya KumarStephen M King
Feb 9, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gáspár Jékely
Jun 2, 2021·Journal of Cellular Physiology·Vishwanatha K S RaoRichard E Mains
Jun 6, 2021·The FEBS Journal·Nils BäckBetty A Eipper

❮ Previous
Next ❯

Methods Mentioned

BETA
amidation
scanning electron microscopy
ion exchange chromatography
gene knockdown
transmission electron microscopy
GTPase
RNA-seq
electron microscopy
PCR
Chip

Software Mentioned

GeneTools
ImageJ
WMD3
Virtualdub
AxioVision
Metamorph
Cuffdiff
Photoshop

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.