A bioartificial liver support system using primary hepatocytes: a preclinical study in a new porcine hepatectomy model

Surgery
Nils R FrühaufChristoph E Broelsch

Abstract

Bioartificial liver support systems providing a bridge to transplantation or even a definitive treatment of acute hepatic failure are a current focus of research. Different devices are used, although an impact on patient survival is doubtful for the time being. After developing a new flat membrane bioreactor, further preclinical studies have become necessary. Existing animal models of fulminant hepatic failure show common difficulties in defining a reproducible loss of functional liver tissue while considering systemic side effects. We now present a reproducible model with total hepatectomy in pigs, suitable to test the safety and efficacy of liver support systems. Twelve pigs underwent total hepatectomy by using silicone tubes and a Y-adapter as vascular prosthesis; intracranial pressure was measured via a subdural probe. Anhepatic pigs were monitored under general anesthesia until death occurred. All were treated with a new flat membrane bioreactor (FMB) that contained porcine hepatocytes in 6 of the 12 cases. Our hepatectomy technique proved to be successful without requiring venovenous bypass circulation. Mean vascular clamping time was 9 minutes. The mean survival time was longer in animals treated with hepatocyte-equipped...Continue Reading

References

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Citations

Jun 20, 2014·World Journal of Gastroenterology : WJG·Cheng-Bo YuLan-Juan Li
Jul 24, 2010·Critical Care : the Official Journal of the Critical Care Forum·Christian ThielMartin Schenk
Mar 13, 2009·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·M J NellC E J van Rensburg
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Oct 10, 2006·Artificial Organs·Oleksandr Seleverstov, Augustinus Bader
Jul 14, 2010·Artificial Organs·Nikolaos ArkadopoulosVassilios Smyrniotis
Jul 14, 2010·Fundamental & Clinical Pharmacology·Michael MaringkaAugustinus Bader
Nov 18, 2005·The Journal of Surgical Research·Gernot M KaiserChristoph E Broelsch
Apr 22, 2011·Laboratory Animals·N R FrühaufG M Kaiser
Feb 9, 2006·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·R LadurnerA Königsrainer
Dec 31, 2005·Metabolic Brain Disease·Mireille Bélanger, Roger F Butterworth
Jan 1, 2010·Cell Medicine·B AndriaR A F M Chamuleau
Oct 10, 2009·Molecular and Cellular Biochemistry·Shibashish GiriAugustinus Bader

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