A bioassay for NSILA-S in individual serum samples and its relationship to somatotropin

The Journal of Clinical Endocrinology and Metabolism
R C FranklinD P Cameron

Abstract

A practical bioassay for the acid-ethanol soluble non-suppressible insulin-like activity (NSILA-S) of individual serum samples has been developed utilizing the incorporation of 14C-glucose into the lipid faction of isolated adipocytes. NSILA-S activity was correlated with somatotropin status. Thus, the mean potencies (+/-SD) relative to an extract of pooled normal human serum were: normal samples 1.11 +/- 0.14, acromegalic 2.91 +/- 0.72, and somatotropin deficient 0.13 +/- 0.06. This variation in NSILA-S was not due to variability in extraction recoveries. The within assay precision was 9% (coefficient of variation) and the between assay 23%. This method allows the simultaneous extraction and processing of relatively large numbers of samples, and compares favorably with other more complex methods. Because of the evidence that NSILA-S may be related to the somatomedins, the present method should provide a simpler and more reliable alternative to the cartilage bioassays used to measure somatomedin activity.

Citations

Jan 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G H TeschA C Herington
Jan 1, 1983·The International Journal of Biochemistry·H J Cornell, A C Herington
Jan 1, 1983·The International Journal of Biochemistry·A C HeringtonA D Kuffer
Jan 1, 1985·The International Journal of Biochemistry·H J CornellA C Herington
Nov 1, 1979·Molecular and Cellular Endocrinology·R C FranklinA C Herington
Sep 1, 1986·Molecular and Cellular Endocrinology·P Roupas, A C Herington
Jan 1, 1984·Preparative Biochemistry·H J CornellA C Herington
Nov 1, 1979·The British Journal of Dermatology·D S JolliffeI Sarkany
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Aug 1, 1987·Clinical Endocrinology·S SchwarzG Wick

Related Concepts

Acromegaly
Sheehan Syndrome
NSILA-S
Recombinant Growth Hormone

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