A Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures

International Journal of Molecular Sciences
Matteo BecattiMaria Elisabetta Coccia

Abstract

Oxidative stress plays a major role in critical biological processes in human reproduction. However, a reliable and biologically accurate indicator of this condition does not yet exist. On these bases, the aim of this study was to assess and compare the blood and follicular fluid (FF) redox status of 45 infertile subjects (and 45 age-matched controls) undergoing in vitro fertilization (IVF), and explore possible relationships between the assessed redox parameters and IVF outcomes. Reactive Oxygen Species (ROS) production, assessed by flow cytometry analysis in blood leukocytes and granulosa cells, significantly increased (p < 0.05) in infertile patients. Also, oxidative stress markers-ThioBarbituric Acid-Reactive Substances (TBARS) as an index of lipid peroxidation, and Oxygen Radical Absorbance Capacity (ORAC) to account for total antioxidant capacity, both assayed by fluorometric procedures-in blood and FF were significantly (p < 0.001) modified in infertile patients compared to the control group. Moreover, a significant correlation between blood redox markers and FF redox markers was evident. An ORAC/TBARS ratio, defined as the redox index (RI), was obtained in the plasma and FF of the patients and controls. In the patients,...Continue Reading

References

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Citations

Jan 10, 2019·Human Fertility : Journal of the British Fertility Society·Riddhi ThakerSunil Kumar
Jul 25, 2019·Journal of Assisted Reproduction and Genetics·Tiziana BacchettiGianna Ferretti
Dec 12, 2019·Frontiers in Endocrinology·Hiroyuki SasakiMamoru Tanaka
Dec 22, 2019·Antioxidants & Redox Signaling·Lucia von MengdenJohan Smitz
Jun 1, 2018·Reproductive Sciences·Maria Elisabetta CocciaFelice Petraglia
Jul 16, 2020·Journal of the Endocrine Society·Bartira Marques PizarroTania Maria Ortiga-Carvalho
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Martha A Sánchez-Rodríguez, Víctor Manuel Mendoza-Núñez
Sep 10, 2020·Biology·Alexandra Almansa-OrdonezFilippo Zambelli
Sep 26, 2021·Clinical and Experimental Immunology·Alessandra BettiolClaudia Fiorillo

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Methods Mentioned

BETA
blood collection
sedation
Flow Cytometry

Software Mentioned

BD FACSDiva
SPSS ( Statistical Package for Social Sciences

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