Oct 1, 2011

A biochemical fluorometric method for assessing the oxidative properties of HDL

Journal of Lipid Research
Theodoros KelesidisOtto O Yang

Abstract

Most current assays of HDL functional properties are cell-based. We have developed a fluorometric biochemical assay based on the oxidation of dihydrorhodamine 123 (DHR) by HDL. This cell-free assay assesses the intrinsic ability of HDL to be oxidized by measuring increasing fluorescence due to DHR oxidation over time. The assay distinguishes the oxidative potential of HDL taken from different persons, and the results are reproducible. Direct comparison of this measurement correlated well with results obtained using a validated cell-based assay (r(2) = 0.62, P < 0.001). The assay can be scaled from a 96-well format to a 384-well format and, therefore, is suitable for high-throughput implementation. This new fluorometric method offers an inexpensive, accurate, and rapid means for determining the oxidative properties of HDL that is applicable to large-scale clinical studies.

  • References32
  • Citations34

Citations

Mentioned in this Paper

Dihydrorhodamine 123
Fluorometry
Rhodamine 123
Oxidation
Morphologic Artifacts
Pro-Apolipoprotein A-I
Inflammation
Fluorochromes
Oxidation-Reduction
Alpha-1 Lipoprotein

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