A bioinformatic approach to the identification of a conserved domain in a sugarcane legumain that directs GFP to the lytic vacuole

Functional Plant Biology : FPB
Mark A JacksonDonald J Maclean

Abstract

Sugarcane is an ideal candidate as a biofactory for the production of alternate higher value products. One way of achieving this is to direct useful proteins into the vacuoles within the sugarcane storage parenchyma tissue. By bioinformatic analysis of gene sequences from putative sugarcane vacuolar proteins a motif has been identified that displays high conservation across plant legumain homologues that are known to function within vacuolar compartments. This five amino acid motif, represented by the sequence IRLPS in sugarcane is shown to direct an otherwise secreted GFP fusion protein into a large acidic and proteolytic vacuole in sugarcane callus cells as well as in diverse plant species. In mature sugarcane transgenic plants, the stability of GFP appeared to be dependent on cell type, suggesting that the vacuolar environment can be hostile to introduced proteins. This targeting motif will be a valuable tool for engineering plants such as sugarcane for production of novel products.

References

Nov 25, 1991·Proceedings of the National Academy of Sciences of the United States of America·J M NeuhausT Boller
Jan 1, 1990·The Plant Cell·J DeneckeR Deblaere
Mar 1, 1996·Current Biology : CB·W ChiuJ Sheen
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J HaseloffS Hodge
Apr 24, 1999·The Plant Cell·F Marty
May 14, 1999·Trends in Plant Science·A Vitale, N V Raikhel
Oct 16, 1999·The Plant Cell·G Y JauhJ C Rogers
May 6, 2003·Journal of Experimental Botany·Ricardo FluckigerGian-Pietro Di Sansebastiano
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Enrique RojoNatasha V Raikhel
Aug 9, 2003·The Plant Journal : for Cell and Molecular Biology·Kentaro TamuraIkuko Hara-Nishimura
Dec 19, 2003·Nucleic Acids Research·Neil D RawlingsAlan J Barrett
Jul 7, 2005·Traffic·David G RobinsonGiselbert Hinz
Jul 19, 2005·Transgenic Research·Ming-Li WangHenrik H Albert
Jul 29, 2005·Plant & Cell Physiology·Claude Saint-Jore-DupasGomord Véronique
Jul 1, 1990·Plant Physiology·G E Welbaum, F C Meinzer
Jul 1, 1992·Plant Cell Reports·J J FinerM D McMullen
May 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D I LastE S Dennis

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Citations

Jun 4, 2019·Frontiers in Plant Science·Mark A JacksonDavid J Craik
Aug 27, 2010·Plant Molecular Biology·Maurice BoschVernonica E Franklin-Tong

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