A Biological and Immunological Characterization of Schistosoma Japonicum Heat Shock Proteins 40 and 90α.

International Journal of Molecular Sciences
Zhipeng XuHong You

Abstract

We characterized Schistosoma japonicum HSP40 (Sjp40) and HSP90α (Sjp90α) in this study. Western blot analysis revealed both are present in soluble egg antigens and egg secretory proteins, implicating them in triggering the host immune response after secretion from eggs into host tissues. These observations were confirmed by immunolocalization showing both HSPs are located in the Reynolds' layer within mature eggs, suggesting they are secreted by miracidia and accumulate between the envelope and the eggshell. Both HSPs are present in the musculature and parenchyma of adult males and in the vitelline cells of females; only Sjp90α is present on the tegument of adults. Sjp40 was able to enhance the expression of macrophages, dendritic cells, and eosinophilic cells in mouse liver non-parenchymal cells, whereas rSjp90α only stimulated the expression of dendritic cells. T helper 1 (Th1), Th2, and Th17 responses were increased upon rSjp40 stimulation in vitro, but rSjp90 only stimulated an increased Th17 response. Sjp40 has an important role in reducing the expression of fibrogenic gene markers in hepatic stellate cells in vitro. Overall, these findings provide new information on HSPs in S. japonicum, improving our understanding of the...Continue Reading

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Citations

Aug 7, 2021·Pathogens·Boontarikaan WititkornkulRussell M Morphew

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Methods Mentioned

BETA
PCR
flow cytometry
amidation
glycosylation
enzyme-linked immunosorbent assay
PMA
FACS

Software Mentioned

GraphPad
FlowJo
BioEdit
TreeStar
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Scan Prosite
Rotor Gene 6000 series
MODEL
ExPASy
MEGA

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