A biologically active rhIGF-1 fusion accumulated in transgenic rice seeds can reduce blood glucose in diabetic mice via oral delivery

Peptides
Tingting XieDaichang Yang

Abstract

Human insulin-like growth factor 1(hIGF-1) is essential for cell proliferation and used therapeutically in treating various diseases including diabetes mellitus. Here, we present that a recombinant hIGF-1(rhIGF-1) was expressed fused with the C-terminus of a rice luminal binding protein and accumulated highly in rice seeds, reaching 6.8+/-0.5% of total seed protein. The rhIGF-1 fusion was demonstrated to possess biological activity to stimulate cell proliferation. Importantly, the unprocessed transgenic seeds could significantly increase plasma rhIGF-1 level and reduce blood glucose of diabetic mice via oral delivery. Further studies suggested that transgenic seeds reduced blood glucose of diabetic mice by enhancing islet cells survival and increasing insulin secretion rather than increasing insulin sensitivity. These results indicated the potential of the novel fusion expression system in production and oral delivery of biologically active small peptides for diseases.

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Citations

Nov 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yang HeDaichang Yang
Jul 13, 2011·International Journal of Molecular Sciences·Agnieszka SirkoPatrycja Redkiewicz
Feb 26, 2013·International Journal of Molecular Sciences·Na AnDaichang Yang
Jan 15, 2014·Plant Cell Reports·Jiquan OuDaichnag Yang
Aug 3, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yao ShengWentao Xu
Feb 5, 2013·Journal of Biotechnology·Liping ZhangDaichang Yang
Oct 1, 2011·Biotechnology Advances·Parvaiz AhmadF Al-Qurainy
Sep 20, 2011·Biotechnology Advances·Jianfeng XuPamela J Weathers
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Jan 1, 2016·Applied Biochemistry and Microbiology·M S BurlakovskiyL A Lutova
Aug 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Evangelia VamvakaTeresa Capell

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