A bioluminescent arsenite biosensor designed for inline water analyzer

Environmental Science and Pollution Research International
Sandra PrévéralDaniel Garcia

Abstract

Whole-cell biosensors based on the reporter gene system can offer rapid detection of trace levels of organic or metallic compounds in water. They are well characterized in laboratory conditions, but their transfer into technological devices for the surveillance of water networks remains at a conceptual level. The development of a semi-autonomous inline water analyzer stumbles across the conservation of the bacterial biosensors over a period of time compatible with the autonomy requested by the end-user while maintaining a satisfactory sensitivity, specificity, and time response. We focused here on assessing the effect of lyophilization on two biosensors based on the reporter gene system and hosted in Escherichia coli. The reporter gene used here is the entire bacterial luciferase lux operon (luxCDABE) for an autonomous bioluminescence emission without the need to add any substrate. In the cell-survival biosensor that is used to determine the overall fitness of the bacteria when mixed with the water sample, lux expression is driven by a constitutive E. coli promoter PrpoD. In the arsenite biosensor, the arsenite-inducible promoter P ars involved in arsenite resistance in E. coli controls lux expression. Evaluation of the shelf l...Continue Reading

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Citations

Feb 24, 2016·Environmental Science and Pollution Research International·Nathalie FrancheMireille Ansaldi
Nov 14, 2016·Environmental Science and Pollution Research International·Elodie C T DescampsNicolas Ginet
May 14, 2020·Journal of Environmental Health Science & Engineering·Evrim Elcin, Huseyin Avni Öktem
Jun 18, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nadine Lobsiger, Wendelin J Stark
May 10, 2020·Applied and Environmental Microbiology·Anissa DieudonneDavid Pignol
Oct 27, 2015·Environmental Science and Pollution Research International·Julien CayronAgnès Rodrigue
May 29, 2016·Environmental Science and Pollution Research International·Catherine BrutescoNicolas Ginet
Mar 19, 2021·Chemical Society Reviews·Aisha J Syed, James C Anderson
Jun 8, 2021·Biosensors & Bioelectronics·Michael MoraskieSylvia Daunert

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