A Bioluminescent Probe for Imaging Endogenous Peroxynitrite in Living Cells and Mice

Analytical Chemistry
Jun-Bin LiXiaobing Zhang

Abstract

Peroxynitrite (ONOO-), an extremely reactive nitrogen species (RNS), is implicated in diverse pathophysiological conditions, including cancer, neurodegenerative diseases, and inflammation. Sensing and imaging of ONOO- in living systems remains challenging due to the high autofluorescence and the limited light penetration depth. In this work, we developed a bioluminescent probe BP-PN, based on luciferase-luciferin pairs and the ONOO--responded group α-ketoamide, for highly sensitive detection and imaging of endogenous ONOO- in living cells and mice for the first time. Attributed to the BL without external excitation, the probe BP-PN exhibits a high signal-to-noise ratio with relatively low autofluorescence. Furthermore, we examine the application of the probe BP-PN using the mice model of inflammation, and BP-PN shows high sensitivity for imaging endogenous ONOO- in inflamed mice. This newly developed bioluminescent probe would be a potentially useful tool for in vivo imaging of ONOO- in wider physiological and pathological processes.

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Oct 12, 2018·Chemical Communications : Chem Comm·Hong Huang, Yang Tian
Nov 24, 2018·Organic & Biomolecular Chemistry·Chunchao TangMinyong Li
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