A biomimetic collagen/heparin multi-layered porous hydroxyapatite orbital implant for in vivo vascularization studies on the chicken chorioallantoic membrane

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Kai JinJuan Ye

Abstract

The vascularization of an orbital implant is a key issue for reducing complications, such as exposure and infection. Here, we developed a facile layer-by-layer assembly approach to modify porous hydroxyapatite (pHA) orbital implants with five collagen (COL)/heparin (HEP) multilayers. SEM characterization showed that the average pore size of the pHA/(COL/HEP)5 scaffold was 316.8 ± 77.1 μm. After being coated with five COL/HEP multilayers, the mechanical strength was improved compared with that of the pHA scaffolds. The in vitro assay displayed that the pHA scaffolds covered with COL/HEP multilayers resulted in a larger number of human umbilical vein endothelial cells after being cultured for 14 days. The macroscopic evaluation and semi-quantitative vascular density analysis of the chicken chorioallantoic membrane assay showed that the pHA/(COL/HEP)5 scaffolds resulted in more intense angiogenesis than the pHA scaffolds. These studies demonstrate that the biomembrane-mimicking coating of COL/HEP multilayers is a simple and effective strategy to endow combined biological performances of pHA orbital implants and to potentially reduce implant-related complications.

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Citations

Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Francesco Baino, Isabel Potestio
Jan 13, 2017·Journal of Tissue Engineering and Regenerative Medicine·Alexander KöwitschThomas Groth
May 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jing YangWencheng Zhang
Jul 11, 2018·Colloids and Surfaces. A, Physicochemical and Engineering Aspects·David A Castilla-CasadiegoJorge Almodovar
Jun 10, 2019·ACS Biomaterials Science & Engineering·David A Castilla-CasadiegoJorge Almodovar

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