DOI: 10.1101/488494Dec 5, 2018Paper

A biosafety level-2 dose-dependent lethal mouse model of spotted fever rickettsiosis: Rickettsia parkeri Atlantic Rainforest-like isolate

BioRxiv : the Preprint Server for Biology
Andrés Felipe Londoño


Background The species of the Rickettsia genus is separated into four groups: the ancestral group, typhus group, transitional group and spotted fever group. Rickettsia parkeri, a spotted fever group Rickettsia, has been reported across the American continents as infecting several tick species and is associated with a relatively mild human disease characterized by eschar formation at the tick feeding site, fever, myalgia and rash. Currently several mouse models that provide a good approach to study the acute lethal disease caused by Rickettsia, but these models can only be performed in an animal biosafety level 3 laboratory. We present an alternative mouse model for acute lethal rickettsial disease, using R. parkeri and C3H/HeN mice, with the advantage that this model can be studied in an animal biosafety level 2 laboratory. Principal findings In the C3H/HeN mouse model, we determined that infection with 1x106 and 1x107 viable R. parkeri Atlantic Rainforest-like isolate produced dose-dependent severity, whereas infection with 1x108 viable bacteria resulted in a lethal illness. The animals became moribund on day five or six post-infection. The lethal disease was characterized by ruffled fur, erythema, labored breathing, decreased...Continue Reading

Related Concepts

Acute Disease
Labor (Childbirth)
Rickettsia Infections

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

The American Journal of Pathology
Tais B SaitoDavid H Walker
Infection and Immunity
Britton J GraspergeKevin R Macaluso
Emerging Infectious Diseases
William J H McBrideDrew Wenck
Emerging Infectious Diseases
Timothy J WhitmanJohn W Sanders
Journal of the Mississippi State Medical Association
Okechukwu EkennaJerome Goddard
© 2021 Meta ULC. All rights reserved