A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water

Enzyme and Microbial Technology
Fernando Campanhã VicentiniOrlando Fatibello-Filho

Abstract

In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry in the presence of catechol. The determination of catechol was carried out by amperometry and presented a linear concentration range from 2.5×10(-6) to 9.5×10(-5)molL(-1) with a detection limit of 1.7×10(-7)molL(-1). The developed biosensor showed good repeatability and stability. Moreover, this novel amperometric method was successfully applied in the determination of catechol in natural water samples. The results were in agreement with a 95% confidence level for those obtained using the official spectrophotometric method.

References

Oct 1, 1994·Analytical Biochemistry·R S BrownJ H Luong
Nov 6, 2003·International Journal of Biological Macromolecules·Senem KiralpYusuf Yağci
Jan 13, 2006·Analytical and Bioanalytical Chemistry·Christine M Welch, Richard G Compton
Nov 23, 2006·Journal of Biotechnology·Sanket TembeS F D'Souza
Jan 27, 2009·Chemical Society Reviews·Ulf HanefeldEdmond Magner
Sep 11, 2009·Journal of Colloid and Interface Science·Yu-Chin LinJing-Jong Shyue
Feb 24, 2010·Biosensors & Bioelectronics·Daniela FiorentinoAntonia Mallardi
May 1, 1996·Analytical Chemistry·E BurestedtG Marko-Varga

❮ Previous
Next ❯

Citations

Mar 29, 2019·Biotechnology and Applied Biochemistry·Nasim MalekiNahid Shahabadi
May 28, 2019·Environmental Science and Pollution Research International·Shivamurthy Ravindra YashasNingappa Kumara Swamy
Mar 14, 2020·International Journal of Biological Macromolecules·Abdelmageed M Othman, Ulla Wollenberger
Jun 3, 2021·International Journal of Molecular Sciences·Alexandra Virginia Bounegru, Constantin Apetrei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.