A biosensor for measuring NAD+ levels at the point of care

Nature Metabolism
Qiuliyang YuKai Johnsson

Abstract

The cellular level of nicotinamide adenine dinucleotide (NAD+), through its different functions, affects cellular metabolism and signalling1-3. A decrease in the NAD+ content has been associated with various pathologies and physiological aging4,5, while strategies to boost cellular NAD+ levels have been shown to be effective against age-related diseases in many animal models6. The link between decreased NAD+ levels and numerous pathologies and physiological aging has triggered the need for a simple quantification method for NAD+, ideally applicable at the point of care. Here, we introduce a bioluminescent biosensor for the rapid quantification of NAD+ levels in biological samples, which can be used either in laboratories or at the point of care. The biosensor is a semisynthetic, light-emitting sensor protein that changes the colour of emitted light from blue to red on binding of NAD+. This NAD+-dependent colour change enables the use of the biosensor in paper-based assays in which NAD+ is quantified by measuring the colour of the emitted light by using either a simple digital camera or a plate reader. We used the approach to quantify NAD+ levels in cell culture, tissue and blood samples, yielding results that agreed with those ...Continue Reading

References

Jan 1, 1983·Analytical Biochemistry·M T KarpT N Lövgren
Dec 1, 1983·European Journal of Biochemistry·E M AlgarR S Holmes
Dec 7, 2002·Nature Biotechnology·Antje KepplerKai Johnsson
Dec 13, 2006·Trends in Biochemical Sciences·Peter BelenkyCharles Brenner
Jun 7, 2008·ACS Chemical Biology·Georgyi V LosKeith V Wood
Jun 14, 2008·Genes & Development·Danica ChenLeonard Guarente
Sep 21, 2012·Science Translational Medicine·Nira R PollockGeorge M Whitesides
Jun 9, 2014·Nature Chemical Biology·Rudolf GrissKai Johnsson
Sep 26, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karim GarianiJohan Auwerx
Oct 11, 2016·Nature Communications·Samuel A J TrammellCharles Brenner
Aug 9, 2017·The EMBO Journal·Elena Katsyuba, Johan Auwerx
Sep 14, 2017·Trends in Molecular Medicine·Evandro F FangVilhelm A Bohr
Dec 19, 2017·Cell Metabolism·Jun YoshinoShin-Ichiro Imai
Mar 8, 2018·Cell Metabolism·Luis RajmanDavid A Sinclair

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Citations

Jul 1, 2020·European Journal of Clinical Investigation·Baeki E KangDongryeol Ryu
Aug 5, 2020·Annals of Neurology·Steffen TiedtMartin Dichgans
Jul 23, 2020·Nature Metabolism·Elena KatsyubaJohan Auwerx
Sep 25, 2020·Chembiochem : a European Journal of Chemical Biology·Misaki KawaseTsuyoshi Takahashi
Nov 27, 2020·Biochemical Society Transactions·Lieuwe BiewengaMaarten Merkx
Jan 28, 2021·Nature·Alfredo Quijano-RubioDavid Baker
Aug 17, 2020·Cell Chemical Biology·Anna C Love, Jennifer A Prescher
Jul 1, 2020·Trends in Biochemical Sciences·Xiaolu A Cambronne, W Lee Kraus
Apr 4, 2021·Cells·Ann-Katrin Hopp, Michael O Hottiger
Feb 21, 2021·Aging·Inés Domingo-OrtíAntonio Pineda-Lucena
Dec 10, 2020·Trends in Biotechnology·Alexander Gräwe, Viktor Stein
Oct 15, 2020·ACS Sensors·Hope Adamson, Lars J C Jeuken

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Methods Mentioned

BETA
biosensor
BRET
biosensors
Assay
PCR

Software Mentioned

MultiQuant
s Analyst
Sciex

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