A biosensor platform for rapid detection of E. coli in drinking water

Enzyme and Microbial Technology
Nikou HesariMorteza Abbaszadegan

Abstract

There remains a need for rapid, specific and sensitive assays for the detection of bacterial indicators for water quality monitoring. In this study, a strategy for rapid detection of Escherichia coli in drinking water has been developed. This strategy is based on the use of the substrate 4-methylumbelliferyl-β-d-glucuronide (MUG), which is hydrolyzed rapidly by the action of E. coli β-d-glucuronidase (GUD) enzyme to yield a fluorogenic 4-methylumbelliferone (4-MU) product that can be quantified and related to the number of E. coli cells present in water samples. In this study, the detection time required for the biosensor response ranged between 20 and 120 min, depending on the number of bacteria in the sample. This approach does not need extensive sample processing with a rapid detection capability. The specificity of the MUG substrate was examined in both, pure cultures of non-target bacterial genera such as Klebsiella, Salmonella, Enterobacter and Bacillus. Non-target substrates that included 4-methylumbelliferyl-β-d-galactopyranoside (MUGal) and l-leucine β-naphthylamide aminopeptidase (LLβ-N) were also investigated to identify nonspecific patterns of enzymatic activities in E. coli. GUD activity was found to be specific fo...Continue Reading

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Citations

Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laura PalaUrs Spitz
Jun 28, 2018·Frontiers in Pharmacology·Meerambika MishraDhananjay Yadav
Mar 27, 2021·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Hisashi SatohReiko Hirano
Sep 8, 2018·Analytical Chemistry·Amrita DasSuri S Iyer

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