A biphasic scaffold design combined with cell sheet technology for simultaneous regeneration of alveolar bone/periodontal ligament complex

Biomaterials
Cedryck VaquetteSaso Ivanovski

Abstract

This study describes the design of a biphasic scaffold composed of a Fused Deposition Modeling scaffold (bone compartment) and an electrospun membrane (periodontal compartment) for periodontal regeneration. In order to achieve simultaneous alveolar bone and periodontal ligament regeneration a cell-based strategy was carried out by combining osteoblast culture in the bone compartment and placement of multiple periodontal ligament (PDL) cell sheets on the electrospun membrane. In vitro data showed that the osteoblasts formed mineralized matrix in the bone compartment after 21 days in culture and that the PDL cell sheet harvesting did not induce significant cell death. The cell-seeded biphasic scaffolds were placed onto a dentin block and implanted for 8 weeks in an athymic rat subcutaneous model. The scaffolds were analyzed by μCT, immunohistochemistry and histology. In the bone compartment, a more intense ALP staining was obtained following seeding with osteoblasts, confirming the μCT results which showed higher mineralization density for these scaffolds. A thin mineralized cementum-like tissue was deposited on the dentin surface for the scaffolds incorporating the multiple PDL cell sheets, as observed by H&E and Azan staining. ...Continue Reading

References

Jul 1, 1986·Journal of Clinical Periodontology·J GottlowJ Wennström
Jun 23, 2000·Annals of Periodontology·G C Armitage
Apr 28, 2005·Journal of Periodontal Research·Tatsuya AkizukiIsao Ishikawa
Feb 24, 2006·Nature Medicine·Nicolas L'HeureuxTodd N McAllister
May 25, 2006·Biomaterials·Kee Woei Ng, Dietmar Werner Hutmacher
Jun 10, 2006·International Journal of Oral and Maxillofacial Surgery·H ChimJ-T Schantz
Nov 2, 2006·Journal of Periodontal Research·S C ChenP M Bartold
Jul 17, 2007·Journal of Bioscience and Bioengineering·Rikako KinoMasanobu Munekata
Jan 22, 2008·Journal of Periodontal Research·M Gomez FloresI Ishikawa
Apr 30, 2008·Journal of Biomedical Materials Research. Part a·Jeffrey P SpalazziHelen H Lu
Dec 2, 2008·Journal of Clinical Periodontology·Mara Gomez FloresIsao Ishikawa
Sep 10, 2009·Australian Dental Journal·S Ivanovski
Jul 14, 2010·Journal of Clinical Periodontology·Takanori IwataIsao Ishikawa
Nov 5, 2010·Biomacromolecules·Denver C SurraoBrian G Amsden
Dec 8, 2010·International Orthopaedics·Johannes C ReichertDietmar W Hutmacher
Dec 29, 2010·Tissue Engineering. Part a·Lei WangYan Jin
Jan 25, 2011·Tissue Engineering. Part C, Methods·Samer SroujiErella Livne
Feb 12, 2011·Journal of Tissue Engineering and Regenerative Medicine·J C ReichertD W Hutmacher
Mar 5, 2011·Acta Biomaterialia·Cedryck Vaquette, Justin John Cooper-White
Sep 3, 2011·Biomaterials·Emily C Carlo ReisJohn E Davies
Oct 14, 2011·Biomaterials·Chan Ho ParkWilliam V Giannobile
Nov 19, 2011·Advanced Materials·Toby D BrownDietmar W Hutmacher

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Citations

Aug 27, 2013·Biomacromolecules·Yongqing XiaJian Ren Lu
Jun 19, 2014·BioResearch Open Access·Doris M CamposBrigitte Grosgogeat
Apr 4, 2014·Journal of the Royal Society, Interface·June E JeonDietmar W Hutmacher
May 28, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Wen LiaoNaoyuki Matsumoto
Nov 6, 2013·Tissue Engineering. Part C, Methods·Chan Ho ParkWilliam V Giannobile
Jun 27, 2015·Expert Review of Medical Devices·Christina N M RyanDimitrios I Zeugolis
Apr 23, 2015·Journal of Periodontal Research·P M BartoldD W Hutmacher
Aug 21, 2012·Acta Biomaterialia·Cédryck Vaquette, Justin Cooper-White
Jun 15, 2012·Biomaterials·Fa-Ming ChenQing Yu
Jul 11, 2013·International Journal of Experimental Pathology·Osnat HakimiAndrew Carr
Dec 18, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Takanori IwataTeruo Okano
Dec 7, 2013·Journal of Clinical Periodontology·Pedro F CostaDietmar W Hutmacher
Jul 25, 2013·Journal of Tissue Engineering and Regenerative Medicine·Takanori IwataTeruo Okano
Mar 25, 2016·Current Opinion in Biotechnology·Nancy LeeHelen Lu
May 16, 2014·Journal of Biomedical Materials Research. Part a·Ying GuoJian Yang
May 15, 2013·Journal of Tissue Engineering and Regenerative Medicine·Lorenzo MoroniPamela Habibovic
Jul 3, 2015·Acta Biomaterialia·S M GiannitelliA Rainer
May 26, 2015·Critical Reviews in Biotechnology·Xi ChenYimin Zhao
Nov 5, 2013·Acta Biomaterialia·S M GiannitelliA Rainer
Jul 1, 2015·Journal of Dental Research·F ObregonL E Bertassoni
Feb 28, 2017·Advanced Healthcare Materials·Asha MathewSaso Ivanovski
Sep 13, 2014·Journal of Dental Research·C H ParkY J Seol
Oct 2, 2014·Journal of Dental Research·A FaragS Ivanovski
Aug 21, 2014·Journal of Dental Research·J H KimH W Kim
Aug 21, 2014·Journal of Dental Research·S IvanovskiP M Bartold
Jan 1, 2016·Nanomaterials·Manila ChieruzziStefano Eramo
Mar 4, 2018·Journal of Clinical Periodontology·Amro FaragSaso Ivanovski
Jan 16, 2018·MRS Communications·Alexander J BoysLara A Estroff
Nov 17, 2017·Journal of the Korean Association of Oral and Maxillofacial Surgeons·Laura GaviriaJoo L Ong
Aug 28, 2018·Advanced Healthcare Materials·Cedryck VaquetteSaso Ivanovski
Mar 3, 2018·Tissue Engineering. Part a·Kaoru WashioIsao Ishikawa
Apr 26, 2019·Stem Cells and Development·Ana-Maria SeciuOtilia Zarnescu
Sep 10, 2014·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Kunio KawanishiTeruo Okano
Sep 8, 2019·International Journal of Molecular Sciences·Chan Ho Park
Dec 4, 2019·Journal of Biomedical Materials Research. Part a·Jeong Eun SongGilson Khang
May 30, 2020·International Journal of Molecular Sciences·Joo-Young ParkJeong-Ho Yun

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