A bird's eye view tracking slow nanometer-scale movements of single molecular nano-assemblies.

Methods in Enzymology
Nicole MichelottiNils G Walter

Abstract

Recent improvements in methods of single-particle fluorescence tracking have permitted detailed studies of molecular motion on the nanometer scale. In a quest to introduce these tools to the burgeoning field of DNA nanotechnology, we have exploited fluorescence imaging with one-nanometer accuracy (FIONA) and single-molecule high-resolution colocalization (SHREC) to monitor the diffusive behavior of synthetic molecular walkers, dubbed "spiders," at the single-molecule level. Here we discuss the imaging methods used, results from tracking individual spiders on pseudo-one-dimensional surfaces, and some of the unique experimental challenges presented by the low velocities (approximately 3 nm/min) of these nanowalkers. These experiments demonstrate the promise of fluorescent particle tracking as a tool for the detailed characterization of synthetic molecular nanosystems at the single-molecule level.

Citations

Dec 14, 2011·Computational and Mathematical Methods in Medicine·Chunyan YaoHouxiang Zhang
Apr 11, 2018·Nature Nanotechnology·Julián ValeroMichael Famulok
Dec 2, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Nicole MichelottiNils G Walter
Oct 21, 2016·Nature Protocols·Jinglin FuHao Yan
Jun 23, 2015·Nature Biotechnology·Alexander Johnson-BuckNils G Walter
Sep 22, 2019·Nature Communications·Krishna C SuddalaNils G Walter
Feb 6, 2020·Angewandte Chemie·Alisina BazrafshanKhalid Salaita
May 8, 2018·Nature Nanotechnology·Jieming LiNils G Walter
Mar 12, 2021·ACS Nano·Shuoxing JiangYan Liu
May 25, 2011·ACS Nano·Girish C ShuklaBruce A Shapiro
May 16, 2014·Accounts of Chemical Research·Roman TsukanovEyal Nir
May 17, 2014·ACS Nano·Alexander Johnson-BuckNils G Walter

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