A bispecific antibody based assay shows potential for detecting tuberculosis in resource constrained laboratory settings.

PloS One
Susmita SarkarMavanur R Suresh

Abstract

The re-emergence of tuberculosis (TB) as a global public health threat highlights the necessity of rapid, simple and inexpensive point-of-care detection of the disease. Early diagnosis of TB is vital not only for preventing the spread of the disease but also for timely initiation of treatment. The later in turn will reduce the possible emergence of multi-drug resistant strains of Mycobacterium tuberculosis. Lipoarabinomannan (LAM) is an important non-protein antigen of the bacterial cell wall, which is found to be present in different body fluids of infected patients including blood, urine and sputum. We have developed a bispecific monoclonal antibody with predetermined specificities towards the LAM antigen and a reporter molecule horseradish peroxidase (HRPO). The developed antibody was subsequently used to design a simple low cost immunoswab based assay to detect LAM antigen. The limit of detection for spiked synthetic LAM was found to be 5.0 ng/ml (bovine urine), 0.5 ng/ml (rabbit serum) and 0.005 ng/ml (saline) and that for bacterial LAM from M. tuberculosis H37Rv was found to be 0.5 ng/ml (rabbit serum). The assay was evaluated with 21 stored clinical serum samples (14 were positive and 7 were negative in terms of anti-LAM...Continue Reading

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Citations

Jan 17, 2014·Postgraduate Medical Journal·Pronoti SarkarBarnali Kakati
Oct 8, 2013·Trends in Biotechnology·Hannah ByrneRichard J O'Kennedy
Nov 12, 2013·Diagnostic Microbiology and Infectious Disease·Advaita GangulyHoon H Sunwoo
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Feb 19, 2019·ERJ Open Research·Margarida Correia-NevesGunilla Källenius
Dec 12, 2018·International Journal of Biological Macromolecules·Jyoti GuptaM Saleemuddin
May 25, 2021·Frontiers in Immunology·Jiabing MaCheng Qian

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Methods Mentioned

BETA
nucleic acid amplification
enzyme-linked immunosorbent assay
ELISA
florescence-activated cell sorting
dot blot
FACS
chemical treatment
chemical treatments

Software Mentioned

ASSURED
Excel

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