A block of endocytosis of the yeast cell wall integrity sensors Wsc1 and Wsc2 results in reduced fitness in vivo.

Molecular Genetics and Genomics : MGG
Sabrina WilkJürgen J Heinisch

Abstract

The response to cell surface stress in yeast is mediated by a set of five plasma membrane sensors. We here address the relation of intracellular localization of the sensors Wsc1, Wsc2, and Mid2 to their turnover and signaling function. Growth competition experiments indicate that Wsc2 plays an important role in addition to Wsc1 and Mid2. The two Wsc sensors appear at the bud neck during cytokinesis and employ different routes of endocytosis, which govern their turnover. Whereas Wsc1 uses a clathrin-dependent NPFDD signal, Wsc2 relies on a specific lysine residue (K495). In end3 and doa4 endocytosis mutants, both sensors accumulate at the plasma membrane, and a hypersensitivity to cell wall-specific drugs and to treatment with zymolyase is observed. A haploid strain in which endocytosis of the two sensors is specifically blocked displays a reduced fitness in growth competition experiments. If the Mid2 sensor is mobilized by the addition of an endocytosis signal, it mimics the dynamic distribution of the Wsc sensors, but is unable to complement the specific growth defects of a wsc1 deletion. These data suggest that sensor distribution is not the major determinant for its specificity.

References

Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·J VernaR Ballester
May 18, 1999·Molecular and Cellular Biology·M RajavelD E Levin
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·J M Galan, M Peter
Oct 6, 1999·The Journal of Cell Biology·P A Delley, M N Hall
Aug 8, 2002·FEMS Microbiology Reviews·Frans M KlisStanley Brul
Jul 9, 2003·The Journal of Cell Biology·Mitsuhiro AbeYoshikazu Ohya
Sep 19, 2003·Current Biology : CB·Javier Valdez-Taubas, Hugh R B Pelham
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Oct 27, 2006·Molecular Biology of the Cell·Hai Lan PiaoGregory S Payne
Aug 2, 2007·Molecular Systems Biology·Gavin C Conant, Kenneth H Wolfe
Sep 11, 2007·Fungal Genetics and Biology : FG & B·Rosaura RodicioJürgen J Heinisch
Aug 27, 2009·Molecular Genetics and Genomics : MGG·Arne JendretzkiJürgen J Heinisch
Sep 22, 2009·Nature Chemical Biology·Vincent DupresYves F Dufrêne
Nov 24, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Vincent DupresYves F Dufrêne

❮ Previous
Next ❯

Citations

Oct 19, 2012·Journal of Cell Science·Jürgen J HeinischYves F Dufrêne
Feb 4, 2014·European Journal of Cell Biology·Katja BackhausJürgen J Heinisch
Jun 2, 2011·European Journal of Cell Biology·Katja BackhausJürgen J Heinisch
Jun 7, 2011·European Journal of Cell Biology·Arne JendretzkiJürgen J Heinisch
Jun 7, 2011·Fungal Biology·Brian D ShawSrijana Upadhyay
Jul 17, 2016·Fungal Genetics and Biology : FG & B·Dorthe Rippert, Jürgen J Heinisch
Jun 24, 2016·Cellular Microbiology·Christian KockJürgen J Heinisch
Nov 24, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Vincent DupresYves F Dufrêne
Mar 20, 2016·Applied and Environmental Microbiology·Sirikarn CharoenbhakdiChoowong Auesukaree
Nov 16, 2014·Applied and Environmental Microbiology·Christian KockJürgen J Heinisch
Oct 27, 2017·FEMS Microbiology Reviews·Jürgen J Heinisch, Rosaura Rodicio
Nov 19, 2018·Biophysics Reviews·Kenneth S Gerien, Jian-Qiu Wu
Jul 10, 2020·G3 : Genes - Genomes - Genetics·Vladimir Vélez-SegarraJosé R Rodríguez-Medina
Sep 11, 2019·FEMS Microbiology Reviews·Alexandros AthanasopoulosChristos Gournas
Feb 9, 2019·G3 : Genes - Genomes - Genetics·Ednalise Santiago-CartagenaJosé R Rodríguez-Medina
Sep 2, 2010·ACS Nano·Vincent DupresJürgen J Heinisch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

© 2021 Meta ULC. All rights reserved