A Bordetella pertussis MgtC homolog plays a role in the intracellular survival

PloS One
Juan Hilario CafieroMaria E Rodriguez

Abstract

Bordetella pertussis, the causative agent of whooping cough, has the capability to survive inside the host cells. This process requires efficient adaptation of the pathogen to the intracellular environment and the associated stress. Among the proteins produced by the intracellular B. pertussis we identified a protein (BP0414) that shares homology with MgtC, a protein which was previously shown to be involved in the intracellular survival of other pathogens. To explore if BP0414 plays a role in B. pertussis intracellular survival a mutant strain defective in the production of this protein was constructed. Using standard in vitro growth conditions we found that BP0414 is required for B. pertussis growth under low magnesium availability or low pH, two environmental conditions that this pathogen might face within the host cell. Intracellular survival studies showed that MgtC is indeed involved in B. pertussis viability inside the macrophages. The use of bafilomycin A1, which inhibits phagosome acidification, abolished the survival defect of the mgtC deficient mutant strain suggesting that in intracellular B. pertussis the role of MgtC protein is mainly related to the bacterial adaptation to the acidic conditions found inside the of...Continue Reading

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Citations

Jan 10, 2021·Communications Biology·Alex Rivera-MillotFrançoise Jacob-Dubuisson
Nov 13, 2020·Frontiers in Microbiology·Israel RiveraEric T Harvill
Mar 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Roberto E BrunaMauricio H Pontes

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Datasets Mentioned

BETA
PXD002997

Methods Mentioned

BETA
PCR
fluorescence microscopy
PMA
confocal microscopy
electrophoresis

Software Mentioned

Skyline
Clustal Omega

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