A Bowman-Birk trypsin inhibitor with antiproliferative activity from Hokkaido large black soybeans

Journal of Peptide Science : an Official Publication of the European Peptide Society
Vincent S M Ho, T B Ng

Abstract

A trypsin inhibitor, with an N-terminal sequence highly homologous to those of 8-kDa Bowman-Birk trypsin inhibitors, was isolated from the seeds of Hokkaido large black soybeans. The trypsin inhibitor was unadsorbed on SP-Sepharose but adsorbed on DEAE-cellulose and Mono Q. It inhibited proliferation in breast cancer (MCF-7) cells and hepatoma (Hep G2) cells with an IC50 of 35 and 140 microM, respectively. The trypsin inhibitory activity of the inhibitor was completely preserved after exposure to temperatures up to 100 degrees C for 30 min and to the pH range 2-13 for the same duration. The trypsin inhibitor inhibited HIV-1 reverse transcriptase with an IC50 of 38 microM, but was devoid of antifungal activity toward Fusarium oxysporum and Mycosphaerella arachidicola.

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Citations

Jan 12, 2013·Applied Biochemistry and Biotechnology·Yau Sang ChanTzi Bun Ng
Apr 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Xiu Juan Ye, Tzi Bun Ng
Apr 16, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Xiuli Dan, Tzi Bun Ng
Nov 21, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sze-Kwan Lam, Tzi-Bun Ng
Nov 14, 2015·Critical Reviews in Food Science and Nutrition·Blanca Hernández-Ledesma, Chia-Chien Hsieh
Jul 19, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Yau Sang ChanTzi Bun Ng
Dec 18, 2008·Acta Biochimica Et Biophysica Sinica·Peng LinTb Ng
Mar 23, 2018·Journal of Chromatographic Science·Uttam K RoyBirthe V Nielsen
Sep 26, 2020·Molecular Nutrition & Food Research·Samuel Fernández-Tomé, Blanca Hernández-Ledesma
Mar 12, 2011·ACS Applied Materials & Interfaces·Xinggui GuDaoben Zhu

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