PMID: 9185162Jul 1, 1997Paper

A brain-derived neurotrophic factor (BDNF) related system is involved in the maintenance of the polyinnervate Torpedo electric organ

Neurochemistry International
J M CanalsJ Marsal

Abstract

Target-derived molecules are essential for the maintenance of neuron survival. In the present work, we introduce the electric organ of Torpedo marmorata as a tool for the study of trophic interactions in a polyinervate system. This electric organ maintains a large number of cholinergic terminals on the postsynaptic cell surface. We have observed that a soluble extract derived from the electric organ induces the maturation of Xenopus oocytes injected with presynaptic plasma membranes (PSPM), indicating that a trophic system may exist. Moreover, we have detected a p75NGFR related protein in PSPM by Western blot analysis. These results suggest the presence of a neurotrophin-related system maintaining the polyinnervate electric organ. Furthermore, molecular experiments showed that the brain-derived neurotrophic factor (BDNF) is the neurotrophin operating in our model. Using degenerate oligonucleotides which comprise a conserved fragment of all neurotrophins, we have only amplified by polymerase chain reaction a BDNF fragment. In a similar way, we have amplified and cloned a fragment of the TrkB/C high affinity BDNF receptor. The fact that degenerate oligonucleotides only amplify BDNF allows us to conclude that the polyinnervation i...Continue Reading

References

May 1, 1975·Developmental Biology·P C Letourneau
May 1, 1992·Journal of Neuroscience Research·A LamP A Ponte
Mar 23, 1990·Science·P C MaisonpierreG D Yancopoulos
Jul 1, 1989·Trends in Neurosciences·R W Oppenheim
Jun 1, 1989·Neuron·Y A Barde
Sep 4, 1987·Science·R Levi-Montalcini
Jul 22, 1983·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R MilediK Sumikawa
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
May 1, 1994·Trends in Neurosciences·R M LindsayP S DiStefano
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·J MarsalR Miledi
Nov 1, 1994·Journal of Neurobiology·M V Chao
Dec 1, 1995·Current Opinion in Cell Biology·M C Birling, J Price

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Citations

Mar 10, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G Heinrich, T Lum

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